Hepatic Arterial Perfusion Is Essential for the Spontaneous Recovery From Focal Hepatic Venous Outflow Obstruction in Rats
Hepatic Arterial Perfusion Is Essential for the Spontaneous Recovery From Focal Hepatic Venous Outflow Obstruction in Rats
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DOI：
10.1111/j.1600-6143.2011.03682.x
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发表时间：
2011-11-01
影响因子：
8.8
通讯作者：
Dahmen, U.
中科院分区：
文献类型：
--
作者：
Huang, H.;Deng, M.;Dahmen, U.
我们之前观察到，在大鼠门静脉高灌注模型中，局灶性肝静脉流出梗阻通过窦状管的形成自发恢复。我们的目的是研究肝动脉灌注不足是否会加重肝实质损伤，减缓恢复速度，并影响局灶性肝静脉流出梗阻后窦状管的形成。结扎供体右肝正中静脉后，分别行动脉化和非动脉化同种肝移植。评估肝损伤、微循环、再生和血管重构。在动脉化的受者中，术后一天（POD）可以看到融合性坏死穿插着存活的肝细胞门周岛，血管化的窦状管可见血流，被正常的窦状结构包围。肝实质的完全恢复是通过坏死的吸收和肝细胞的增殖来建立的，在存活的门脉岛和边界区可见。肝动脉灌注不足导致梗阻区完全坏死，门静脉周围区无活肝细胞。肝细胞增殖仅在边界区可见。在POD28上，瘢痕样区可见灌注血管结构，周围无正常正弦结构。肝动脉灌注决定局灶性肝静脉流出梗阻后肝坏死程度、血管化窦状管形成及实质恢复情况。
We previously observed that focal hepatic venous outflow obstruction recovered spontaneously by the formation of sinusoidal canals in a rat model of portal hyperperfusion. We aimed to investigate whether the lack of hepatic arterial perfusion aggravates parenchymal damage, decelerates recovery and influences the formation of sinusoidal canals after focal hepatic venous outflow obstruction. Rats were subjected to arterialized versus nonarterialized syngeneic liver transplantation after ligating the right median hepatic vein in the donor. Hepatic damage, microcirculation, regeneration and vascular remodeling were evaluated. In arterialized-recipients, confluent necrosis interspersed with viable periportal islands of hepatocytes, and vascularized sinusoidal canals with visible blood flow, surrounded by normal sinusoidal structure, were visible on postoperative day (POD) 2. Complete parenchymal recovery was consequently established by resorption of necrosis and hepatocyte proliferation, detected in viable portal islands and border zone. Lack of hepatic arterial perfusion caused complete necrosis in the obstruction zone without viable hepatocytes in the periportal area on POD2. Hepatocyte proliferation was only visible in the border zone. On POD28, perfused vascular structures, without neighboring normal sinusoidal structures, were observed in the scar-like area. Hepatic arterial perfusion determined the extent of hepatic necrosis, the formation of vascularized sinusoidal canals and the parenchymal recovery, after focal hepatic venous outflow obstruction.