CD9 suppresses human extravillous trophoblast invasion
CD9 suppresses human extravillous trophoblast invasion
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DOI：
10.1016/j.placenta.2016.09.014
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发表时间：
2016-11-01
期刊：
影响因子：
3.8
通讯作者：
Fujiwara, Hiroshi
中科院分区：
文献类型：
--
作者：
Matsumoto, Hisanori;Sato, Yukiyasu;Fujiwara, Hiroshi
在人类胎盘形成过程中，绒毛外滋养层细胞（EVT）侵入母体蜕膜并重建母体螺旋动脉。然而，控制EVT行为的精确机制尚未详细阐明。据报道，CD 9是细胞运动相关分子。由于我们先前观察到CD 9在人EVT上表达，我们检测了CD 9在EVT侵袭过程中的可能参与。胎盘和脐带样本来自接受法律的流产、正常分娩或子宫切除术的患者。通过免疫细胞化学染色、流式细胞术和RT-PCR检测植入部位和从绒毛外植体培养物分离的EVT、EVT衍生的永生化细胞系Swan 71和HUVEC上的CD 9表达。沿着shRNAmir基因敲低处理后，用基质胶侵袭实验检测抗CD 9功能性抗体（ALB 6）对EVT和Swan 71细胞侵袭能力的影响。CD 9在EVT浸润和血管内的交界处呈高表达。ALB 6和shRNAmir处理均能促进EVT和Swan 71细胞的侵袭。缺氧条件下Swan 71细胞表面CD 9表达降低，而与HUVEC共培养后其表达增加。这些发现表明，CD 9可以减弱EVT的氧气环境和母体内皮细胞的影响下，入侵，提出CD 9是一个潜在的调节人胎盘形成。(C)2016爱思唯尔有限公司版权所有。
During human placentation, the extravillous trophoblast (EVT) invades the maternal decidua and reconstructs maternal spiral arteries. However, the precise mechanisms that control EVT behavior have not yet been elucidated in detail. CD9 has been reported to be a cell-motility-related molecule. Since we previously observed that CD9 was expressed on human EVT, we examined the possible involvement of CD9 in the invasion process of EVT. Placental and umbilical samples were obtained from patients who underwent legal abortions, normal delivery, or hysterectomy. The expression of CD9 at the implantation site and on isolated EVT from a villous explant culture, an EVT-derived immortalized cell line, Swan71, and HUVEC was examined by immunocytochemical staining, flow cytometry, and RT-PCR. The effects of anti-CD9 functional antibody (ALB6) on EVT and Swan71 cell invasion were further examined by matrigel invasion assay along with shRNAmir gene knockdown treatment. CD9 was highly expressed on EVT at the boundary region of EVT invasion and intravascular EVT. EVT and Swan71 cell invasions were promoted by ALB6 or shRNAmir treatment. CD9 expression on Swan71 cells was reduced under hypo oxygenic conditions, while its expression was increased by the co-culture with HUVEC. These findings suggest that CD9 could attenuate EVT invasion under the influence of an oxygen environment and maternal endothelial cells, proposing that CD9 is a potential regulator of human placental formation. (C) 2016 Elsevier Ltd. All rights reserved.