Comparison of particle sizing techniques in the case of inhalation dry powders
Comparison of particle sizing techniques in the case of inhalation dry powders
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DOI：
10.1002/jps.1154.abs
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发表时间：
2001-12-01
影响因子：
3.8
通讯作者：
Vanbever, R
中科院分区：
文献类型：
--
作者：
Bosquillon, C;Lombry, C;Vanbever, R
这项工作的目的是(i)验证用于分析吸入干粉情况下初级粒度的电区传感和激光衍射，以及（ii）研究粉末的聚集状态对施胶技术的影响。用白蛋白、乳糖和双棕榈酰磷脂酰胆碱的混合物喷雾干燥制备了自由流动的干粉。用甘露醇代替乳糖，去除白蛋白，在高相对湿度下雾化，都增加了粉末的凝聚力。通过光学显微镜和电子显微镜收集的主要颗粒尺寸进行了自动测量比较。通过电区传感获得的质量模式和在压力为3 bar的压缩空气中分散或在水中悬浮后用激光衍射测量的质量中值直径，以及在功率为60 W的超声分散30 s下测量的质量中值直径，均提供了与显微镜测量值接近的初级粒径。然而，这些条件只适用于轻微到适度聚集的粉末。对于强团聚的粉末，只有在湿态激光衍射结合超声分散的情况下才能精确测量粉末的尺寸。我们的研究表明，初级粒度的测量高度依赖于粉末材料和适当的颗粒分散。(C) 2001.中国医药科学杂志（英文版）。
The objectives of this work were (i) to validate electrical zone sensing and laser diffraction for the analysis of primary particle size in the case of inhalation dry powders and (ii) to study the influence of the aggregation state of the powder on the sizing techniques. Free-flowing dry powders were prepared by spray-drying with a combination of albumin, lactose, and dipalmitoylphosphatidylcholine. The replacement of lactose by mannitol, the removal of albumin, and the atomization at high relative humidity all increased powder cohesion. Automated measurements were compared with primary particle sizes collected by light and electron microscopy. The mass mode obtained by electrical zone sensing and the mass median diameter measured by laser diffraction following dispersion with compressed air at a pressure of 3 bar or following suspension in water and ultrasonic dispersion at a power of 60 W for 30 s each provided primary particle sizes close to microscopy measurements. However, these conditions only applied in the case of slightly to moderately aggregated powders. For strongly agglomerated powders, an exact measurement of the size was only collected by laser diffraction in the wet state combined with ultrasonic dispersion. Our study underlies how measurement of primary particle size highly depends on both powder material and proper particle dispersion. (C) 2001 Wiley-Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 90:2032-2041, 2001.