Changes in pulsatile secretion patterns of LH, FSH, progesterone, androstenedione and oestradiol in cows after superovulation with PMSG.
Changes in pulsatile secretion patterns of LH, FSH, progesterone, androstenedione and oestradiol in cows after superovulation with PMSG.
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PMSG 超数排卵后奶牛 LH、FSH、黄体酮、雄烯二酮和雌二醇脉动分泌模式的变化。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
J. V. D. Broek
中科院分区：
文献类型：
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作者：
M. Bevers;S. Dieleman;H. V. Tol;D. M. Blankenstein;J. V. D. Broek
6头小母牛经肌肉注射。用2500 i.u. 48 h后给予PMSG 15 mg前列腺素。在第10天（PMSG给药后7小时）、黄体消退期间（前列腺素给药后7小时）和之后的第二天，通过尾腔静脉中的导管每10分钟采集一次连续血样，持续8小时。四个正常周期的小母牛作为对照组。测量腔静脉样本中孕酮、雄烯二酮、雌二醇、LH、FSH和PMSG的浓度，并估计除PMSG外的所有激素的发作脉冲的频率和振幅。在黄体退化开始后50小时通过卵巢切除术收集卵巢，以确定排卵前卵泡（大于或等于10 mm的非闭锁卵泡）的数量。通过给予PMSG刺激卵泡生长导致对类固醇和内源性促性腺激素分泌的以下影响。(1)孕酮浓度和发作性脉冲的幅度和频率没有改变。平均值（+/- s.e.m.）第10天（L）、黄体消退期间（LR）和之后一天（F）的浓度分别为54.1 +/- 5.8、19.1 +/- 3.1和3.4 +/- 0.9 nmol/l。(2)雄烯二酮的间歇性分泌模式没有改变。L、LR和F期的平均浓度分别为0.20 +/- 0.02、0.15 +/- 0.02和0.11 +/- 0.02 nmol/l。(3)雌二醇浓度从L期的17.1 +/- 3.0 pmol/l增加至F期的233.7 +/- 86.4 pmol/l。与对照动物的相应时期相比，脉冲幅度增强，而脉冲频率保持不变。雌二醇浓度与排卵前卵泡数呈显著正相关（r = 0.82，P <0.05）。(4)与对照小母牛的相应时期（分别为9.5 +/- 0.9和7.0 +/- 1.5）相比，LR（3.2 +/- 0.7）和F（4.3 +/- 0.4）期间的发作性LH脉冲频率（/8 h）受到抑制。排卵前LH峰值发生在4 6治疗小母牛。(5)在LR和F期，FSH浓度、脉冲幅度和频率均受到抑制（17.4 +/- 0.9 mg/l、4.7 +/- 0.8 μ g/l和7.5 +/- 0.4脉冲/8 h）周期与相应的F周期值相比（35.6 +/- 6.2 mg/l、9.8 +/- 1.6 μ g/l和9.3 +/- 0.3脉冲/8 h）。（400字处截断摘要）
Six heifers were injected i.m. with 2500 i.u. PMSG followed by 15 mg prostaglandin 48 h later. Serial blood samples were collected through a catheter in the caudal vena cava every 10 min for 8 h on Day 10 (7 h after PMSG administration), during luteal regression (7 h after prostaglandin administration) and on the day thereafter. Four normally cyclic heifers served as a control group. Concentrations of progesterone, androstenedione, oestradiol, LH, FSH, and PMSG in the vena cava samples were measured and the frequency and amplitudes of episodic pulses of all hormones were estimated except for PMSG. Ovaries were collected by ovariectomy at 50 h after onset of luteal regression to determine the number of preovulatory follicles (non-atretic follicles greater than or equal to 10 mm). Stimulation of follicular growth by administration of PMSG resulted in the following effects on the secretion of steroids and endogenous gonadotrophins. (1) There were no alterations in progesterone concentration and the amplitude and frequency of episodic pulses. Mean (+/- s.e.m.) concentrations were 54.1 +/- 5.8, 19.1 +/- 3.1 and 3.4 +/- 0.9 nmol/l on Day 10 (L), during luteal regression (LR) and on the day thereafter (F) respectively. (2) There were no alterations in the episodic secretion patterns of androstenedione. Mean concentrations were 0.20 +/- 0.02, 0.15 +/- 0.02 and 0.11 +/- 0.02 nmol/l for the L, LR and F periods respectively. (3) There was an increase in oestradiol concentration from 17.1 +/- 3.0 pmol/l during the L period to 233.7 +/- 86.4 pmol/l during the F period. Pulse amplitude was enhanced compared to corresponding periods in control animals whereas pulse frequency remained the same. The oestradiol concentration was significantly correlated with the number of preovulatory follicles (r = 0.82, P less than 0.05). (4) There was a suppression of the frequency of episodic LH pulses (/8 h) during the LR (3.2 +/- 0.7) and F (4.3 +/- 0.4) periods compared to corresponding periods in control heifers (9.5 +/- 0.9 and 7.0 +/- 1.5 respectively). The preovulatory LH peak occurred earlier in 4 of 6 treated heifers. (5) There was a suppression of FSH concentrations, pulse amplitude and frequency during the LR and F (17.4 +/- 0.9 mg/l, 4.7 +/- 0.8 microgram/l and 7.5 +/- 0.4 pulses/8 h) periods compared to the corresponding F-period values (35.6 +/- 6.2 mg/l, 9.8 +/- 1.6 micrograms/l and 9.3 +/- 0.3 pulses/8 h) in control heifers.(ABSTRACT TRUNCATED AT 400 WORDS)
促性腺激素的血浆浓度、排卵前卵泡发育和黄体功能与牛卵泡液引起的小母牛发情延迟相关。
DOI：
10.1530/jrf.0.0760609
发表时间：
1986
期刊：
Journal of reproduction and fertility
影响因子：
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作者：
Quirk,SM;Fortune,JE
通讯作者：
Fortune,JE