Physical localization of the mesoderm development (mesd) functional region.
Physical localization of the mesoderm development (mesd) functional region.
复制标题
中胚层发育 (mesd) 功能区域的物理定位。
DOI：
10.1006/geno.2000.6264
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发表时间：
2000
期刊：
影响因子：
4.4
通讯作者：
Holdener,BC
中科院分区：
文献类型：
--
作者：
Wines,ME;Shi,Y;Lindor,M;Holdener,BC
中胚层发育（mesd）的功能间隔是原条形成和中胚层诱导的必要条件。Mesd定义为7号染色体上重叠的白化病（c）缺失。我们已经构建了一个细菌人工染色体（BAC）重叠群，跨越mesd功能区。BAC末端序列识别识别新表达序列的三个片段。在重叠群中，来自Del（7）Tyrc-3 YPSd和Del（7）Tyrc-112 K的近端断裂点的定位定义了310-350 kb的缺失间隔，这对mesd功能是必需的。重要的是，使用BAC转基因拯救，我们定义了一个75 kb的mesd关键区域，其中包含至少一个表达序列。
The mesoderm development (mesd) functional interval is essential for primitive streak formation and mesoderm induction. Mesd is defined by overlapping albino (c) deletions on chromosome 7. We have constructed a bacterial artificial chromosome (BAC) contig that spans the mesd functional region. BAC end-sequence identifies three segments that recognize novel expressed sequences. Localization of the proximal breakpoints from Del(7)Tyrc−3YPSdand Del(7)Tyrc−112Kwithin the contig defines a deletion interval of 310–350 kb that is essential for mesd function. Importantly, using BAC transgene rescue, we define a 75-kb mesd critical region containing at least one expressed sequence.