Choline partially prevents the impact of ethanol on the lipid raft dependent functions of l1 cell adhesion molecule.
Choline partially prevents the impact of ethanol on the lipid raft dependent functions of l1 cell adhesion molecule.
复制标题
胆碱部分阻止乙醇对 l1 细胞粘附分子的脂筏依赖性功能的影响。
DOI：
10.1111/acer.12554
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Bearer,CynthiaF
中科院分区：
文献类型：
--
作者：
Tang,Ningfeng;Bamford,Penny;Jones,Jace;He,Min;Kane,MaureenA;Mooney,SandraM;Bearer,CynthiaF
BackgroundFetal alcohol spectrum disorder, the leading known cause of mental retardation, is caused by alcohol exposure during pregnancy. One mechanism of ethanol (EtOH) teratogenicity is the disruption of the functions of L1 cell adhesion molecule (L1). These functions include enhancement of neurite outgrowth, trafficking through lipid rafts, and signal transduction. Recent data have shown that choline supplementation of rat pups reduces the effects of EtOH on neurobehavior. We sought to determine whether choline could prevent the effect of EtOH on L1 function using a simple experimental system.MethodsCerebellar granule neurons (CGN) from postnatal day 6 rat pups were cultured with and without supplemental choline, and the effects on L1 signaling, lipid raft distribution, and neurite outgrowth were measured in the presence or absence of EtOH.ResultsCholine significantly reduced the effect of EtOH on L1 signaling, the distribution of L1 in lipid rafts and L1‐mediated neurite outgrowth. However, choline supplemented EtOH‐exposed cultures remained significantly different than controls.ConclusionsCholine pretreatment of CGN significantly reduces the disruption of L1 function by EtOH, but does not completely return L1 function to baseline. This experimental system will enable discovery of the mechanism of the neuroprotective effect of choline.
