Hybrid Synthetic-Biological Hydrogel System for Adipose Tissue Regeneration
Hybrid Synthetic-Biological Hydrogel System for Adipose Tissue Regeneration
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DOI：
10.1002/mabi.201800122
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发表时间：
2018-11-01
影响因子：
4.6
通讯作者：
Hayes, Daniel J.
中科院分区：
文献类型：
--
作者：
Li, Shue;Poche, John Nicholas;Hayes, Daniel J.
水凝胶是一种很有前途的脂肪组织再生支架。目前，采用生物活性分子掺入水凝胶体系，可以提高基质干细胞的细胞增殖速率或改善成脂分化性能，但由于采用光/热固化进行聚合，往往存在较高的费用或细胞毒性。在本研究中，脱细胞脂肪组织以不同浓度与巯基丙烯酸酯部分结合，然后通过迈克尔加成反应聚合生成水凝胶。结果表明，分离的脂肪来源的细胞外基质（ECM）的主要成分是胶原I. ECM的机械性能不受ECM掺入的负面影响聚乙二醇（PEG）。此外，人脂肪来源的干细胞（hASCs）被包裹在ECM PEG水凝胶中，ECM浓度从0%到1%不等。结果表明，在成脂条件下培养的hASCs在1% ECM PEG水凝胶中保持最高的活力和增殖率，形成的脂质最多。此外，在体内研究中，与其他ECM含量较低的ECM PEG水凝胶相比，1% ECM组在第14天观察到更多的脂肪再生。综上所述，这些发现表明ECM聚乙二醇水凝胶是一种很有前途的脂肪组织再生替代品。
Hydrogels are promising scaffolds for adipose tissue regeneration. Currently, the incorporation of bioactive molecules in hydrogel system is used, which can increase the cell proliferation rate or improve adipogenic differentiation performance of stromal stem cells but often suffers from high expense or cytotoxicity because of light/thermal curing used for polymerization. In this study, decellularized adipose tissue is incorporated, at varying concentrations, with a thiol-acrylate fraction that is then polymerized to produce hydrogels via a Michael addition reaction. The results reveal that the major component of isolated adipose-derived extracellular matrix (ECM) is Collagen I. Mechanical properties of ECM polyethylene glycol (PEG) are not negatively affected by the incorporation of ECM. Additionally, human adipose-derived stem cells (hASCs) are encapsulated in ECM PEG hydrogel with ECM concentrations varying from 0% to 1%. The results indicate that hASCs maintained the highest viability and proliferation rate in 1% ECM PEG hydrogel with most lipids formation when cultured in adipogenic conditions. Furthermore, more adipose regeneration is observed in 1% ECM group with in vivo study by Day 14 compared to other ECM PEG hydrogels with lower ECM content. Taken together, these findings suggest the ECM PEG hydrogel is a promising substitute for adipose tissue regeneration applications.