Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis
Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis
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DOI：
10.1039/b604542a
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发表时间：
2006-01-01
期刊：
影响因子：
6.1
通讯作者：
Wilhelm, Claire
中科院分区：
文献类型：
--
作者：
Pamme, Nicole;Wilhelm, Claire
分离活细胞的能力是细胞研究的一个重要方面。磁性细胞分离方法是体细胞分离中最有效的方法之一。随着生物技术领域的微流控平台的发展，小型化磁细胞分选机的设计是可取的。在这里，我们报道了在微流控磁分离装置中装载磁性纳米颗粒的细胞的连续分选。细胞通过微流控室，并通过磁场使其偏离流动方向。研究了小鼠巨噬细胞和人卵巢癌细胞（HeLa细胞）两种类型的细胞。偏转取决于磁矩和细胞的大小以及施加的流速。实验观测到的挠度与计算结果吻合较好。此外，使用相同的微流体装置演示了磁性和非磁性细胞的分离。
The ability to separate living cells is an essential aspect of cell research. Magnetic cell separation methods are among some of the most efficient methods for bulk cell separation. With the development of microfluidic platforms within the biotechnology sector, the design of miniaturised magnetic cell sorters is desirable. Here, we report the continuous sorting of cells loaded with magnetic nanoparticles in a microfluidic magnetic separation device. Cells were passed through a microfluidic chamber and were deflected from the direction of flow by means of a magnetic field. Two types of cells were studied, mouse macrophages and human ovarian cancer cells ( HeLa cells). The deflection was dependent on the magnetic moment and size of the cells as well as on the applied flow rate. The experimentally observed deflection matched well with calculations. Furthermore, the separation of magnetic and non-magnetic cells was demonstrated using the same microfluidic device.