Observation of symmetric denaturation of hemoglobin subunits by electrospray ionization mass spectrometry.
Observation of symmetric denaturation of hemoglobin subunits by electrospray ionization mass spectrometry.
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通过电喷雾电离质谱观察血红蛋白亚基的对称变性。
DOI：
10.1002/jms.1843
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Jianfeng Liu
中科院分区：
文献类型：
--
作者：
Xian Wang;Wenjie Zhao;Xin Lin;Bo Su;Jianfeng Liu
采用电喷雾质谱（ESI-MS）研究了溶剂对猪血红蛋白（Hb）变性的影响。这项工作为Hb变性的对称性提供了证据，并证明了在溶液中加入酸和有机共溶剂时，α-和β-单体的血红素损失平行发生。当受到以下解决方案条件之一时（pH3.2 -4.0或15-30%乙腈-水或30-45%甲醇-水溶液）中，α-和β-球蛋白发生对称解离释放血红素基团，ESI-MS检测到。圆二色谱（CD）和荧光光谱（FS）数据表明，酸诱导和有机溶剂诱导的血红素释放，如在质谱中观察到的，可能归因于蛋白质中发生的构象变化的不同方面。溶剂的酸度对二级结构有显著影响，而溶液中的有机物含量水平（15-30%乙腈或30-45%甲醇）倾向于破坏Hb球蛋白的三级结构，两者都导致血红素从每个亚基释放。
Electrospray ionization mass spectrometry (ESI-MS) has been used to characterize the denaturation of porcine hemoglobin (Hb) induced by solvent changes. This work provides evidence for the symmetric nature of Hb denaturation and demonstrates that heme losses from α- and β-monomers occur in parallel, in response to the addition of acid and organic co-solvents in solution. When subject to one of the following solution conditions (pH 3.2-4.0 or 15-30% acetonitrile-water or 30-45% methanol-water solution), α- and β-globins undergo symmetric dissociation to release the heme groups, which is detected by ESI-MS. Circular dichroism (CD) and fluorescence spectroscopy (FS) data show that the acid-induced and organic solvent-induced heme release, as observed in the mass spectra, can probably be ascribed to different aspects of the conformational changes taking place in the protein. The acidity of the solvent has a significant effect on the secondary structure, whereas organic content level in solution (15-30% acetonitrile or 30-45% methanol) tends to destroy the tertiary structure of Hb globins, both leading to release of the heme from each subunit.
影响因子：
2.9
作者：
Griffith, WP;Kaltashov, IA
通讯作者：
Kaltashov, IA