Effect of light/dark regimen on N-nitrosoethylurea-induced transplacental carcinogenesis in rats
Effect of light/dark regimen on N-nitrosoethylurea-induced transplacental carcinogenesis in rats
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DOI：
10.1016/s0304-3835(00)00673-x
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发表时间：
2001-02-10
期刊：
影响因子：
9.7
通讯作者：
Anisimov, VN
中科院分区：
文献类型：
--
作者：
Beniashvili, DS;Benjamin, S;Anisimov, VN
将怀孕女性随机分为3组（每组24例），分别在12:12 h光照/黑暗方案（1组）、恒定光照（24 h /天，2组）和持续黑暗（3组）。所有大鼠于妊娠第18-19天尾静脉注射n -亚硝基乙基脲（NEU） （80 mg/kg）。分娩后，在三种不同的光照/黑暗方案下，对哺乳期间（分娩后1个月）的泌乳母鼠及其后代进行保存。然后将每组子代分别置于12:12 h光照/黑暗环境下，观察至自然死亡。持续光照显著促进胎盘癌的发生，而持续黑暗则抑制胎盘癌的发生。总肿瘤发生率，包括周围神经系统和肾脏肿瘤的发生率为2.6；恒定光照条件下2组大鼠的存活率分别是1组（12:12 h明暗交替）的2.5倍和8.5倍，显著缩短（P < 0.05）。另一方面，在妊娠期和哺乳期暴露于持续黑暗的环境中，可显著抑制NEU处理大鼠子代的胎盘癌变。与1组（12:12 h明暗对照）相比，3组暴露于黑暗的大鼠周围神经系统总肿瘤、总幽默的发生率分别降低2.4倍和2.7倍，生存时间延长（P < 0.05）。因此，我们的数据首次显示了光-暗方案对NEU诱导大鼠移植胎盘癌变实现的修饰作用。(C) 2001爱思唯尔科学爱尔兰有限公司版权所有。
Pregnant females were randomly subdivided into three groups (24 rates per group) and kept at the 12:12 h light/dark regimen (group 1) 1 at the constant light illumination (24 h a day, group 2) or at the continuous darkness (group 3). N-nitrosoethylurea (NEU) has been injected into the tail vein of all rats (80 mg/kg) on the 18-19th day of the pregnancy. After the delivery the lacting dams and their progeny during the lactation period (1 month after delivery) were kept also at the three different light/dark regimens. Then all offspring from each group was kept at the 12:12 h light/dark regimen, males and females separately, and were observed until natural death.. The exposure to constant light significantly promoted the transplacental carcinogenesis whereas the exposure to constant darkness inhibited it. The incidence of total tumors, tumors of both a peripheral nervous system and kidney was 2.6; 2.5 and 8.5 times higher, and survival significantly shorter, correspondingly, in rats from the group 2 exposed to the constant light regimen as compared to the group 1 (12:12 h light/dark regimen) (P < 0.05). On the other hand, the exposure to the continuous darkness during the pregnancy and the lactation period significantly inhibited the transplacental carcinogenesis in the offspring of rats treated with NEU. The incidence of total tumors, humors of a peripheral nervous system was by 2.4 and 2.7 times less, and survival longer, respectively, in exposed to the darkness rats from the group 3 as compared to the group 1 (12:12 h light/dark regimen) (P < 0.05). Thus, our data firstly have shown the modifying effect of light-dark regimen on the realization of the transplacental carcinogenesis induced by NEU in rats. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.