Gene discovery in the wood-forming tissues of poplar:: Analysis of 5,692 expressed sequence tags
Gene discovery in the wood-forming tissues of poplar:: Analysis of 5,692 expressed sequence tags
复制标题
DOI：
10.1073/pnas.95.22.13330
复制
发表时间：
1998-10-27
影响因子：
11.1
通讯作者：
Lundeberg, J
中科院分区：
文献类型：
--
作者：
Sterky, F;Regan, S;Lundeberg, J
基因组研究的一个快速发展的领域是表达序列标签（est）的产生，其中大量随机选择的cDNA克隆被部分测序。est的收集反映了样本组织中基因表达的水平和复杂性。迄今为止，大多数植物est来自非木本植物，如拟南芥、芸苔、玉米和水稻。在这里，我们展示了从两种杨树，杨树（Populus tremula L, x tremuloides Michx）和杨树（Populus trichocarpa 'Trichobel）的成木组织中大规模生产ESTs。分析的5692个ESTs共代表两个cDNA文库的3719个独特转录本，其中2245个转录本对应820个蛋白质功能。森林生物技术特别感兴趣的是，4%的est参与细胞壁形成的各种过程，如木质素和纤维素合成，5%类似于发育调节因子和已知信号转导途径的成员，2%参与激素生物合成。另外12%的ESTs显示与现有数据库中的任何其他DNA或蛋白质序列没有显著的相似性。公共数据库中缺少这些序列可能表明这些蛋白质在木材形成中的特定作用。cDNA文库及其附带的数据库是森林研究的宝贵资源，有助于了解木材形成的遗传控制，并有助于未来改变木材和纤维的特性以供工业使用。
A rapidly growing area of genome research is the generation of expressed sequence tags (ESTs) in which large numbers of randomly selected cDNA clones are partially sequenced. The collection of ESTs reflects the level and complexity of gene expression in the sampled tissue, To date, the majority of plant ESTs are from nonwoody plants such as Arabidopsis, Brassica, maize, and rice. Here, we present a large-scale production of ESTs from the wood-forming tissues of two poplars, Populus tremula L, x tremuloides Michx, and Populus trichocarpa 'Trichobel.' The 5,692 ESTs analyzed represented a total of 3,719 unique transcripts for the two cDNA libraries, Putative functions could be assigned to 2,245 of these transcripts that corresponded to 820 protein functions. Of specific interest to forest biotechnology are the 4% of ESTs involved in various processes of cell wall formation, such as lignin and cellulose synthesis, 5% similar to developmental regulators and members of known signal transduction pathways, and 2% involved in hormone biosynthesis. An additional 12% of the ESTs show ed no significant similarity to any other DNA or protein sequences in existing databases. The absence of these sequences from public databases may indicate a specific role for these proteins in wood formation. The cDNA libraries and the accompanying database are valuable resources for forest research directed toward understanding the genetic control of wood formation and future endeavors to modify wood and fiber properties for industrial use.