Anatomical distribution of sex steroid hormone receptors in the brain of female medaka
Anatomical distribution of sex steroid hormone receptors in the brain of female medaka
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DOI：
10.1002/cne.23255
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发表时间：
2013-06
影响因子：
2.5
通讯作者：
B. Zempo;S. Kanda;K. Okubo;Y. Akazome;Y. Oka
中科院分区：
文献类型：
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作者：
B. Zempo;S. Kanda;K. Okubo;Y. Akazome;Y. Oka
雌激素和雄激素分别通过雌激素受体（ER）和雄激素受体（AR）在协调生殖功能中发挥重要作用。这些受体被认为对调节下丘脑-垂体-性腺（HPG）轴很重要。尽管它们的生物学重要性，性类固醇受体的分布还没有得到充分的解剖分析在硬骨鱼脑。硬骨鱼有许多特征，这使得对生殖功能的调节机制的理解独特的方法。青鳉是研究类固醇受体介导系统调节机制的良好模型系统，因为1）它们的繁殖条件易于操纵; 2）我们可以利用基因组数据库; 3）分子遗传工具，如转基因技术，是适用的。采用原位杂交技术分析雌青鳉脑内ERα、ERβ1、ERβ2、ARα和ARβ mRNA的分布。我们发现，所有亚型的ER和AR的表达在以下核团：腹侧端脑区（Vd）的背侧部分，腹侧端脑区（Vs）的上连合部分，腹侧端脑区（Vp）的后连合部分，视前区（POA），和结节腹侧核（NVT）。已知这些区域参与性行为（Vd、Vs、Vp、POA）或HPG轴（NVT）的调节。这些表达ER和/或AR的神经元可以根据其轴突投射调节性行为或HPG轴。未来的分析应该针对本研究中描述的神经元，以扩展我们对生殖中枢调控机制的理解。神经学比较杂志521：1760-1780，2013。© 2012 Wiley Periodicals，Inc.
Estrogen and androgen play crucial roles in coordinating reproductive functions through estrogen receptors (ERs) and androgen receptors (ARs), respectively. These receptors are considered important for regulation of the hypothalamo‐pituitary‐gonadal (HPG) axis. Despite their biological importance, the distribution of sex steroid receptors has not been fully analyzed anatomically in the teleost brain. The teleosts have many characteristic features, which allow unique approaches toward an understanding of the regulatory mechanisms of reproductive functions. Medaka serves as a good model system for studying the mechanisms by which steroid receptor‐mediated systems are regulated, because 1) their breeding conditions can be easily manipulated; 2) we can take advantage of the genome database; and 3) molecular genetic tools, such as transgenic techniques, are applicable. We analyzed the distribution of ERα, ERβ1, ERβ2, ARα, and ARβ mRNA by in situ hybridization in the brain of female medaka. We found that all subtypes of ERs and ARs were expressed in the following nuclei: the dorsal part of the ventral telencephalic area (Vd), supracommissural part of the ventral telencephalic area (Vs), postcommissural part of the ventral telencephalic area (Vp), preoptic area (POA), and nucleus ventralis tuberis (NVT). These regions are known to be involved in the regulation of sexual behavior (Vd, Vs, Vp, POA) or the HPG axis (NVT). These ER‐ and/or AR‐expressing neurons may regulate sexual behavior or the HPG axis according to their axonal projections. Future analysis should be targeted to the neurons described in the present study to extend our understanding of the central regulatory mechanisms of reproduction. J. Comp. Neurol. 521:1760–1780, 2013. © 2012 Wiley Periodicals, Inc.