Morphology and increased growth rate of atherosclerotic intimal smooth-muscle cells.
Morphology and increased growth rate of atherosclerotic intimal smooth-muscle cells.
复制标题
动脉粥样硬化内膜平滑肌细胞的形态和生长速度增加。
DOI：
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发表时间：
1988
影响因子：
4.6
通讯作者：
K. Nishida
中科院分区：
文献类型：
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作者：
Y. Yoshida;M. Mitsumata;T. Yamane;M. Tomikawa;K. Nishida
动脉粥样硬化内膜平滑肌细胞（SMCs）在体外表现出更高的生长活性比中膜SMCs从动脉粥样硬化或正常动脉粥样硬化。电镜观察表明，原代培养的内膜平滑肌细胞细胞器丰富，胞浆内细丝较少。它们被认为是合成表型。相反，大多数中膜SMC有丰富的细丝和较少的细胞器。它们被认为是收缩表型。当动脉粥样硬化的内膜和正常的中膜平滑肌细胞接种在I型胶原凝胶上时，细胞质环磷酸腺苷浓度增加，DNA合成受到抑制。在凝胶上培养的内膜平滑肌细胞显示收缩表型。二丁酰环磷酸腺苷加入到培养基中减少DNA合成和改变细胞表型收缩状态。内膜平滑肌细胞对高脂血症低密度脂蛋白和同型半胱氨酸的损伤具有更强的抵抗力。内膜平滑肌细胞溶酶体酶活性增强。
Atherosclerotic intimal smooth-muscle cells (SMCs) in vitro showed higher growth activity than did medial SMCs obtained from either atherosclerotic or normal aortas. Using an electron microscope, it was proved in primary cultures by an explant method that intimal SMCs had rich organelles and fewer filaments in their cytoplasms. They were regarded as synthetic phenotype. In contrast, most medial SMCs had rich filaments and fewer organelles. They were regarded as contractile phenotype. When atherosclerotic intimal and normal medial SMCs were plated on type I collagen gel, cytoplasmic cyclic adenosine monophosphate concentration increased and DNA synthesis was suppressed. Intimal SMCs cultured on the gel showed contractile phenotype. Dibutyryl cyclic adenosine monophosphate added to culture media decreased DNA synthesis and altered cellular phenotype to a contractile state. Intimal SMCs were more resistant to injury by hyperlipidemic low-density liproprotein and homocysteine. Lysosomal enzyme activity was enhanced in intimal SMCs.