Simple surface modification techniques for immobilization of biomolecules on SU-8
Simple surface modification techniques for immobilization of biomolecules on SU-8
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DOI：
10.1007/s10856-008-3471-9
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发表时间：
2009-12-01
影响因子：
3.7
通讯作者：
Mukherji, S.
中科院分区：
文献类型：
--
作者：
Deepu, A.;Sai, V. V. R.;Mukherji, S.
SU-8是一种环氧基负性光致抗蚀剂聚合物，在微加工生物传感器领域有着广泛的应用。SU-8表面需要改性以修饰生物受体。我们研究了在正常实验室条件下通过简单的化学处理接枝所需官能团的可能性。这些化学处理涉及使用预期与酸/碱处理产生的环氧基或羟基反应的交联剂。在这里，使用甘氨酸和11-巯基十一烷酸作为交联剂的表面改性获得的结果的比较。采用碳二亚胺/琥珀酰亚胺化学法将人免疫球蛋白G（HIgG）共价固定在SU-8表面的羧酸上。固定化HIgG的活性通过使用结合到表面的FITC标记的山羊抗HIgG的荧光成像来验证。使用荧光成像来确定最适合用于生物传感器应用的功能化SU-8表面的化学。
SU-8, an epoxy based negative photoresist polymer has found wide range of applications in the field of microfabrication based biosensors. SU-8 surfaces need to be modified in order to immobilize bioreceptors. We studied the possibility of grafting desired functional groups by means of simple chemical treatments under normal laboratory conditions. These chemical treatments involve the use of crosslinkers that are expected to react with epoxy groups or hydroxyl groups generated by acid/alkali treatment. Here, a comparison of the results obtained on surface modification using glycine and 11-mercapto undecanoic acid as crosslinkers is presented. Human Immunoglobin G (HIgG) was covalently immobilized to carboxylic acid on SU-8 surface using carbodiimide/succinimide chemistry. The activity of immobilized HIgG was verified by using fluorescence imaging of FITC tagged goat anti HIgG bound to the surface. Fluorescence imaging was used to determine the chemistry best suited to functionalize SU-8 surface for biosensor applications.