Dietary fish oil reduces survival and impairs bacterial clearance in C3H/Hen mice challenged with Listeria monocytogenes
Dietary fish oil reduces survival and impairs bacterial clearance in C3H/Hen mice challenged with Listeria monocytogenes
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DOI：
10.1042/cs0920095
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发表时间：
1997-01-01
期刊：
影响因子：
6
通讯作者：
Berg, JN
中科院分区：
文献类型：
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作者：
Fritsche, KL;Shahbazian, LM;Berg, JN
1.为了研究膳食脂肪源对宿主抵抗胞内病原体的影响，断奶雌性C3 H/Hen小鼠被喂食三种实验膳食之一，所述三种实验膳食包含20重量%的猪油、大豆油或17重量%的鲱鱼鱼油加3重量%的玉米油。4周后，小鼠的存活（n = 12/治疗组）腹腔内注射2 × 10（6）菌落形成单位的活单核细胞增生李斯特菌。在第二项研究中，在攻击后2、4和7天测定从肝和脾的细菌清除率（n = 8/治疗组），2.我们发现，喂食大豆油或鲱鱼油的小鼠的存活率明显低于喂食猪油的小鼠（P < 0.05），豆油、鲱鱼油和猪油组小鼠的存活率分别为58%（7/12）、33%（4/12）和100%（12/12）。在第4天，喂食鲱鱼鱼油的小鼠在其脾脏中的细菌比喂食猪油或大豆油的小鼠多约1 log（10）（P < 0.001）。总之，膳食脂肪源显著影响鼠对李斯特菌的抗性，其中富含n-3多不饱和脂肪酸的膳食（例如来自鱼油）具有最有害的影响。
1. To investigate the effect of dietary fat source on host resistance to intracellular pathogens, weanling female C3H/Hen mice were fed one of three experimental diets containing, 20% by weight, lard, soybean oil or 17% menhaden fish oil plus 3% corn oil, After 4 weeks, survival of mice (n = 12/treatment group) injected intraperitoneally with 2 x 10(6) colony forming units of live Listeria monocytogenes was determined, In a second study, bacterial clearance from the liver and spleen at 2, 4 and 7 days postchallenge was determined (n = 8/treatment group),2. We found that the survival of mice fed the diets with soybean oil or menhaden fish oil was significantly lower than those fed lard (P < 0.05), Survival rates were 58% (7/12), 33% (4/12) and 100% (12/12), respectively, for mice fed soybean oil, menhaden fish oil and lard, In the second study, mice fed menhaden fish oil had approximately 1 log(10) greater bacteria in their spleens at day 4 than mice fed lard or soybean oil (P < 0.001), There were no significant treatment differences in the number of bacteria recovered from liver samples,3. In summary, dietary fat source significantly affects murine resistance to Listeria, with diets rich in n-3 polyunsaturated fatty acids, such as from fish oil, having the most detrimental effect.