Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA
Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA
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DOI：
10.1073/pnas.2533829100
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发表时间：
2004-01-13
影响因子：
11.1
通讯作者：
Alonso, JC
中科院分区：
文献类型：
--
作者：
Ayora, S;Carrasco, B;Alonso, JC
Bacillus subtilis RecU protein is involved in homologous recombination, DNA repair, and chromosome segregation. Purified RecU binds preferentially to three- and four-strand junctions when compared to single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) (approximate to10- and approximate to40-fold lower efficiency, respectively). RecU cleaves mobile four-way junctions but fails to cleave a linear dsDNA with a putative cognate site, a finding consistent with a similar genetic defect observed for genes classified within the E epistatic group (namely ruvA, recD, and recU). In the presence of Mg2+, RecU also anneals a circular ssDNA and a homologous linear dsDNA with a ssDNA tail and a linear ssDNA and a homologous supercoiled dsDNA substrate. These results suggest that RecU, which cleaves recombination intermediates with high specificity, might also help in their assembly.
