Dual electrospray ion source for electron-transfer dissociation on a hybrid linear ion trap-orbitrap mass spectrometer.
Dual electrospray ion source for electron-transfer dissociation on a hybrid linear ion trap-orbitrap mass spectrometer.
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DOI：
10.1021/ac071444h
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发表时间：
2007-09
影响因子：
7.4
通讯作者：
D. K. Williams;G. McAlister;D. Good;J. Coon;D. Muddiman
中科院分区：
文献类型：
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作者：
D. K. Williams;G. McAlister;D. Good;J. Coon;D. Muddiman
一个双电喷雾电离源（ESI）已被修改，同时产生阳离子和阴离子，从每个发射器，用于执行快速电子转移解离（ETD）离子/离子反应的混合线性离子阱轨道阱质谱仪。与用于产生ETD试剂离子的脉冲双ESI源不同，该源通过物理切换哪一个在大气入口前面来在空间而不是时间上分离发射器。新的安排允许大大增强喷雾稳定性和减少切换时间（<或=30 ms），允许更多的串联质谱每单位时间。在此，我们证明了ETD阴离子群体的稳定性和从多质子化肽阳离子中识别几种C-和Z-型产物离子的能力。
A dual electrospray ionization source (ESI) has been modified to simultaneously produce cations and anions, one from each emitter, for performing rapid electron-transfer dissociation (ETD) ion/ion reactions on a hybrid linear ion trap-orbitrap mass spectrometer. Unlike the pulsed dual ESI sources that were used to generate ETD reagent ions, this source separates the emitters in space, rather than time, by physically switching which one is in front of the atmospheric inlet. The new arrangement allows for substantially enhanced spray stability and decreased switching times (<or=30 ms), allowing for more tandem-MS spectra per unit time. Herein, we demonstrate the stability of the ETD anion population and the ability to identify several c- and z-type product ions from multiply protonated peptide cations.