Chloroplast diversity in the genus Malus:: new insights into the relationship between the European wild apple (Malus sylvestris (L.) Mill.) and the domesticated apple (Malus domestica Borkh.)
Chloroplast diversity in the genus Malus:: new insights into the relationship between the European wild apple (Malus sylvestris (L.) Mill.) and the domesticated apple (Malus domestica Borkh.)
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DOI：
10.1111/j.1365-294x.2006.02924.x
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发表时间：
2006-07-01
影响因子：
4.9
通讯作者：
Roldan-Ruiz, I. .
中科院分区：
文献类型：
--
作者：
Coart, E.;Van Glabeke, S.;Roldan-Ruiz, I. .
为了阐明欧洲野苹果Malus sylvestris（L.）米尔，及其驯化近缘种M.南极洲Borkh.，摘要研究了634份野生和422份来自不同地区的驯化材料的叶绿体DNA变异。M. sylvestris和M.使用10个核微卫星和贝叶斯分配方法对南极洲进行了检查。这使我们能够识别杂交和野生植物逃离种植。68个基因型来自苹果属其它12个野生种，其中M. siversii（Ledeb.）罗姆加入也包括在叶绿体多样性的分析。标记技术的开发类型以前描述的重复和一个新检测到的颠换matK基因。利用PCR-RFLP（Polymerase Chain Reaction-Random Fragment Length Polymorphism）技术进一步研究了叶绿体DNA的变异，并根据所有突变组合构建了单倍型。一个更密切的关系比目前接受的M。sylvestris和M.在细胞质水平上建立了两个物种共有的8个叶绿体单倍型的检测。M. sylvestris和M.在地方一级，在当地栽培品种和同域野生树木之间共享稀有单倍型也很明显。利用野生苹果属基因型在当地栽培苹果的过程中的指示。叶绿体单倍型向M.结果表明，在驯化苹果上发现了3种M.所研究的sieversii树显示了M. sylvestris和M.南极洲令人惊讶的是，M。西佛西树以前被描述为驯化苹果的主要母系祖先。本研究重新开启了关于M.南极洲
To unravel the relationship between the European wild apple, Malus sylvestris (L.) Mill., and its domesticated relative M. domestica Borkh., we studied chloroplast DNA variation in 634 wild and 422 domesticated accessions originating from different regions. Hybridization between M. sylvestris and M. domestica was checked using 10 nuclear microsatellites and a Bayesian assignment approach. This allowed us to identify hybrids and feral plants escaped from cultivation. Sixty-eight genotypes belonging to 12 other wild Malus species, including 20 M. sieversii (Ledeb.) Roem. accessions were also included in the analysis of chloroplast diversity. Marker techniques were developed to type a formerly described duplication and a newly detected transversion in the matK gene. Chloroplast DNA variation was further investigated using PCR-RFLP (Polymerase Chain Reaction-Random Fragment Length Polymorphism), and haplotypes were constructed based on all mutational combinations. A closer relationship than presently accepted between M. sylvestris and M. domestica was established at the cytoplasmic level, with the detection of eight chloroplast haplotypes shared by both species. Hybridization between M. sylvestris and M. domestica was also apparent at the local level with sharing of rare haplotypes among local cultivars and sympatric wild trees. Indications of the use of wild Malus genotypes in the (local) cultivation process of M. domestica and cytoplasmic introgression of chloroplast haplotypes into M. sylvestris from the domesticated apple were found. Only one of the M. sieversii trees studied displayed one of the three main chloroplast haplotypes shared by M. sylvestris and M. domestica. This is surprising as M. sieversii has formerly been described as the main maternal progenitor of the domesticated apple. This study hereby reopens the exciting discussion on the origin of M. domestica.