Endothelin-1 increases intracellular calcium mobilization but not calcium uptake in rabbit vascular smooth muscle cells.
Endothelin-1 increases intracellular calcium mobilization but not calcium uptake in rabbit vascular smooth muscle cells.
复制标题
Endothelin-1 增加兔血管平滑肌细胞的细胞内钙动员，但不增加钙摄取。
DOI：
10.1016/0006-291x(89)91744-0
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Colucci,WS
中科院分区：
文献类型：
--
作者：
Bialecki,RA;IzzoJr,NJ;Colucci,WS
内皮素-1是一种强有力的缩血管肽，它在刺激兔血管平滑肌细胞外钙内流中的作用已被证实。本研究旨在探讨内皮素-1对培养的兔主动脉平滑肌细胞跨膜~（45）Ca ~（2+）内流和细胞内钙动员的影响。在含钙缓冲液中，内皮素-1在10 pM至100 nM范围内诱导浓度依赖性45 Ca 2+外排反应，EC 50约为60 pM。内皮素刺激的最大45 Ca 2+流出量不受细胞外钙缺乏或1 μM维拉帕米的影响。内皮素-1并没有诱导transplasmalemmal 45 Ca 2+摄取的时间长达30分钟。这些研究结果表明，在细胞内钙处理的改变，而不是细胞外钙流入，是负责内皮素刺激的兔主动脉平滑肌细胞内钙浓度的增加。
Conflicting evidence has been reported regarding the role of endothelin-1, a potent vasoconstrictor peptide, in stimulating extracellular calcium influx in rabbit vascular smooth muscle. The objective of this study was to elucidate the effects of endothelin-1 on transmembrane45Ca2+influx and intracellular calcium mobilization in cultured rabbit aortic smooth muscle cells. In calcium containing buffer, endothelin-1 induced a concentration-dependent45Ca2+efflux response over the range of 10 pM to 100 nM with an EC50of approximately 60 pM. Maximum endothelin-stimulated45Ca2+efflux was not affected by the absence of extracellular calcium or the presence of 1 μM verapamil. Endothelin-1 did not induce transplasmalemmal45Ca2+uptake at times up to 30 min. These findings suggest that an alteration in intracellular calcium handling, rather than extracellular calcium influx, is responsible for the endothelin-stimulated increase in intracellular calcium concentration in rabbit aortic smooth muscle cells.