Effects of localized bending on DNA supercoiling.
Effects of localized bending on DNA supercoiling.
复制标题
局部弯曲对 DNA 超螺旋的影响。
DOI：
10.1016/s0968-0004(00)89058-1
复制
发表时间：
1995
影响因子：
13.8
通讯作者：
Olson,WK
中科院分区：
文献类型：
--
作者：
Yang,Y;Westcott,TP;Pedersen,SC;Tobias,I;Olson,WK
DNA双螺旋只有在理想情况下才是直的。实际上，它会弯曲、扭曲和拉伸，以响应局部碱基序列以及与蛋白质和其他结合配体的特异性相互作用。自然发生的弯曲似乎可以促进核小体的组装，并且在某些情况下可以有效地取代体内的调节DNA结合蛋白。最近，一种被称为“有限元分析”的计算方法，工程师经常使用它来分析建筑物和桥梁的稳定性，已被应用于超螺旋DNA结构中自然曲率的定量评估，为这些链的局部，序列依赖性特征和整体拓扑结构之间的关系提供了新的见解。
The DNA double helix is straight only in the idealized case. In reality, it bends, twists and stretches in response to local base sequence and to specific interactions with proteins and other bound ligands. Naturally occurring bends appear to promote the assembly of nucleosomes, and in some cases can effectively replace regulatory DNA-binding proteinsin vivo. Recently, a computational method known as ‘finite element analysis', which is used routinely by engineers to analyse the stability of buildings and bridges, has been applied to the quantitative assessment of natural curvature in supercoiled DNA structures, providing new insight into the relationship between local, sequence-dependent features and the overall topology of these chains.