INHIBITORY EFFECT OF COLCHICINE ON TRANSLOCATION OF ALKALINE-PHOSPHATASE TO THE PLASMA-MEMBRANE CONCOMITANT TO ITS INDUCTION IN RAT-LIVER
INHIBITORY EFFECT OF COLCHICINE ON TRANSLOCATION OF ALKALINE-PHOSPHATASE TO THE PLASMA-MEMBRANE CONCOMITANT TO ITS INDUCTION IN RAT-LIVER
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DOI：
10.1016/0005-2736(81)90465-x
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
IKEHARA, Y
中科院分区：
文献类型：
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作者：
ODA, K;IKEHARA, Y
单次注射秋水仙碱（1-3 mg/kg体重）引起肝碱性磷酸酶的显着诱导，该酶在匀浆中从5-6小时开始线性增加，并在药物治疗后20-22小时达到最大水平（对照活性的14倍）。在质膜中，处理后12小时内比活性的增加和碱性磷酸酶的恢复受到极大抑制。药物治疗与导管结扎联合实验证实了秋水仙碱的这种抑制作用；秋水仙碱也大大抑制了胆管结扎引起的质膜酶升高。每次治疗后8小时测定4组有或没有胆管结扎和/或秋水仙碱给药的大鼠肝脏中酶活性的亚细胞分布。在对照和胆管结扎的肝脏中，在质膜部分中观察到最高的比活性，而在秋水仙碱处理的肝脏中，无论有或没有胆管结扎，在高尔基体部分中发现最高的活性。富含诱导酶的高尔基膜明显被药物阻断，以防止向质膜迁移，从而证明高尔基复合体参与了新合成的碱性磷酸酶向质膜的易位途径。
A single injection of colchicine (1-3 mg/kg body wt) caused a remarkable induction of hepatic alkaline phosphatase, which increased linearly in the homogenate starting at 5-6 h and reached a maximum level (14-fold of the control activity) at 20-22 h after the drug treatment. In the plasma membrane, the increase in specific activity and the recovery of alkaline phosphatase were greatly inhibited up to 12 h after the treatment. Such an inhibitory effect of colchicine was confirmed by a combination experiment of the drug treatment with duct ligation; in the plasma membrane elevation of the enzyme induced by bile duct ligation was also greatly retarded by colchicine. The subcellular distribution of the enzyme activity in livers was determined among the 4 groups of rats with or without bile duct ligation and/or colchicine administration taken at 8 h after each treatment. In the control and the bile duct-ligated livers, the highest specific activity was observed in the plasma membrane fraction, while in the colchicine-treated livers, with or without bile duct ligation, the highest activity was found in the Golgi fractions. The Golgi membranes enriched with the induced enzyme were apparently blocked by the drug to prevent migration toward the plasma membrane, thus demonstrating involvement of the Golgi complex in the translocation route of newly synthesized alkaline phosphatase to the plasma membrane.