Demonstration of vasoproliferative activity from mammalian retina.
Demonstration of vasoproliferative activity from mammalian retina.
复制标题
哺乳动物视网膜血管增殖活性的证明。
DOI：
10.1083/jcb.84.2.298
复制
发表时间：
1980-02
影响因子：
7.8
通讯作者：
Fenselau, A
中科院分区：
文献类型：
--
作者：
Glaser, B M;D'Amore, P A;Michels, R G;Patz, A;Fenselau, A
血管增殖活性已在人、牛和猫来源的视网膜提取物中得到证实。这些视网膜提取物能够刺激（a）培养的牛血管内皮细胞的增殖和胸苷摄取，和（B）鸡绒毛尿囊膜上的新生血管形成。骨骼肌、心肌和肝脏的提取物缺乏类似的刺激活性。该活性不可透析，在56 ℃下稳定，在100 ℃下失活。视网膜提取物刺激角膜成纤维细胞的增殖，但对血管平滑肌细胞的增殖没有影响。间接证据表明，在一些眼部疾病中，血管增生因子从视网膜中释放。本报告中的数据代表了哺乳动物视网膜血管增生活性的第一个直接证明。
Vasoproliferative activity has been demonstrated in extracts of retinas from human, bovine, and feline sources. These retinal extracts are capable of stimulating (a) proliferation and thymidine uptake of bovine vascular endothelial cells in culture and (b) neovascularization on the chick chorioallantoic membrane. Extracts of skeletal muscle, cardiac muscle, and liver lack similar stimulatory activity. The activity is nondialyzable, stable at 56 degrees C, and inactivated at 100 degrees C. Retinal extracts stimulate the proliferation of corneal fibroblasts but have no effect on the proliferation of vascular smooth muscle cells. Indirect evidence suggests the liberation of a vasoproliferative factor from retina in several ocular disorders. The data in this report represent the first direct demonstration of vasoproliferative activity from mammalian retina.