Construction of a dual affinity tagged allele of the Rb1 tumor suppressor gene in the mouse.
Construction of a dual affinity tagged allele of the Rb1 tumor suppressor gene in the mouse.
复制标题
小鼠 Rb1 肿瘤抑制基因的双亲和力标记等位基因的构建。
DOI：
10.1002/dvg.20589
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Goodrich,DavidW
中科院分区：
文献类型：
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作者：
Xiao,Hai;Zhang,Xiaojing;Goodrich,DavidW
Rb 1抑癌基因功能的缺失参与了大多数人类癌症的发生。Rb 1蛋白（Rb 1 protein，pRb）与多种细胞基因和蛋白质结合，通过分子间的相互作用介导Rb 1的多种功能。考虑到这些分子相互作用的潜在复杂性和缺乏已建立的pRb纯化方法，很难系统地鉴定体内不同组织中与肿瘤抑制相关的基因和蛋白质相互作用。为了解决这一限制，我们在小鼠中产生了双亲和力标记的Rb 1等位基因。标记的等位基因作为野生型起作用，并且编码的蛋白质可以通过串联亲和色谱法纯化。该等位基因将促进小鼠中可接近的任何组织中的天然pRb分子相互作用的鉴定和表征。创世纪48：121-126，2010。© 2009 Wiley利斯公司
Loss ofRb1tumor suppressor gene function is involved in the genesis of most human cancers. Novel therapies targetingRb1have been slow to develop because of our incomplete understanding of its molecular mechanisms of action.Rb1protein (pRb) binds a host of cellular genes and proteins, and these molecular interactions mediate its various functions. Given the potential complexity of these molecular interactions and the lack of established methods for pRb purification, it has been difficult to systematically identify gene and protein interactions relevant to tumor suppression in different tissues in vivo. To address this limitation, we have generated a dual affinity taggedRb1allele in the mouse. The tagged allele functions as wild type and the encoded protein can be purified by tandem affinity chromatography. This allele will facilitate identification and characterization of native pRb molecular interactions in any tissue accessible in the mouse. genesis 48:121–126, 2010. © 2009 Wiley‐Liss, Inc.