QTL clusters reflect character associations in wild and cultivated rice
QTL clusters reflect character associations in wild and cultivated rice
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DOI：
10.1007/s00122-001-0819-7
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发表时间：
2002-06-01
影响因子：
5.4
通讯作者：
Morishima, H
中科院分区：
文献类型：
--
作者：
Cai, HW;Morishima, H
基于数量性状位点（qtl）的基因组分布，研究了水稻初级基因库中与分化相关的性状关联的遗传基础。亚洲栽培水稻（Oryza sativa）及其野生祖先（O. rufipogon）的主要进化趋势是：(1)野生型向驯化型的分化（驯化），(2)野生种多年生型和一年生型的生态型分化，(3)栽培种向籼型和粳型的分化。利用一种籼稻品种O. sativa与一株O. rufipogon杂交获得的125个重组自交系（RILs），在12条染色体上定位了147个标记，其中大部分为RFLPs。对37个形态和生理数量性状进行评价，检测到24个性状的qtl。定位的位点显示出由驯化相关性状和籼/粳稻诊断性状的qtl组成的集群的趋势。多年生/一年生类型差异的qtl没有聚类。这种集群现象可以被认为是“多因子联系”，随后是有利于共同适应性状的自然选择。此外，这种聚类现象可能部分是由于一些未知的关键因子通过不同的代谢途径控制各种性状的多效性。发现QTL集群的染色体区域与ril中品种衍生等位基因频率高于预期的基因或基因块所在区域相吻合。这种扭曲的部分原因可能是由于在建立RILs过程中无意识地选择了栽培植物类型。
The genetic basis of character association related to differentiation found in the primary gene pool of rice was investigated based on the genomic distribution of quantitative trait loci (QTLs). Major evolutionary trends in cultivated rice of Asiatic origin (Oryza sativa) and its wild progenitor (O. rufipogon) are: (1) differentiation from wild to domesticated types (domestication), (2) ecotype differentiation between the perennial and annual types in wild races, and (3) the Indica versus Japonica type differentiation in cultivated races. Using 125 recombinant inbred lines (RILs) derived from a cross between an Indica cultivar of O. sativa and a strain of O. rufipogon carrying some Japonica-like characteristics, we mapped 147 markers, mostly RFLPs, on 12 chromosomes. Thirty-seven morphological and physiological quantitative traits were evaluated, and QTLs for 24 traits were detected. The mapped loci showed a tendency to form clusters that are composed of QTLs of the domestication-related traits as well as Indica/Japonica diagnostic traits. QTLs for perennial/annual type differences did not cluster. This cluster phenomenon could be considered "multifactorial linkages" followed by natural selection favoring co-adapted traits. Further, it is possible that the clustering phenomenon is partly due to pleiotropy of some unknown key factor(s) controlling various traits through diverse metabolic pathways. Chromosomal regions where QTL clusters were found coincided with the regions harboring genes or gene blocks where the frequency of cultivar-derived alleles in RILs is higher than expected. This distortion may be partly due to unconscions selection favoring cultivated plant type during the establishment of RILs.