Combined proteomic and functional analysis reveals rich sources of protein diversity in skin mucus and venom from the Scorpaena plumieri fish
Combined proteomic and functional analysis reveals rich sources of protein diversity in skin mucus and venom from the Scorpaena plumieri fish
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DOI：
10.1016/j.jprot.2018.08.002
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发表时间：
2018-09-15
影响因子：
3.3
通讯作者：
Figueiredo, Suely G.
中科院分区：
文献类型：
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作者：
Borges, Marcia H.;Andrich, Filipe;Figueiredo, Suely G.
毒蝎的生物活性可能与毒液和皮肤粘液有关。通过蛋白质组学/功能学方法分析了毒液和皮肤黏液的蛋白质组成和生物活性。共鉴定出885个蛋白，其中毒器提取物（Sp-VAe）中有722个，皮肤粘液提取物（Sp-SMe）中有391个，其中494个仅存在于Sp-VAe中，被命名为S. plumieri Venom proteins (Sp-VP)， 228个在两种提取物中均有发现。所鉴定的许多蛋白质中的大多数与本文报道的生物活性没有直接关系。然而，一些被归类为毒素/潜在的有趣分子：凝集素、蛋白酶和蛋白酶抑制剂在两种提取物中都被检测到，而形成孔的毒素和透明质酸酶与Sp-VP相关。两种提取物都具有蛋白水解和抗微生物活性，而主要的毒性活性——心血管、炎症、溶血和伤害活性——仅由Sp-VAe引起。我们的研究为皮肤粘液和毒液的组成提供了清晰的图像。我们还表明，在中毒观察到的经典效应是由毒腺分子产生的。我们的研究结果为不断增长的scorpaeniform鱼类毒液及其皮肤粘液蛋白添加了新的目录。意义：本研究通过散弹枪蛋白组学方法，在银屑病鱼（Scorpaena plumieri）的有毒器官和皮肤粘液提取物中鉴定了大量的蛋白质，包括经典和非经典毒素。结果表明，毒杀后观察到的毒性作用是由源自毒腺的分子引起的。这些结果为不断增长的scorpaeniform鱼类毒液及其皮肤粘液蛋白的目录增添了新的内容——与陆生动物的毒液和生物活性成分相比，它们几乎没有被探索过。数据可通过ProteomeXchange获得，标识符为PXD009983
The biological activities observed upon envenomation by Scorpaena plumieri could be linked to both the venom and the skin mucus. Through a proteomic/functional approach we analyzed protein composition and biological activities of the venom and skin mucus. We identified 885 proteins: 722 in the Venomous Apparatus extracts (Sp-VAe) and 391 in the Skin Mucus extract (Sp-SMe), with 494 found exclusively in Sp-VAe, being named S. plumieri Venom Proteins (Sp-VP), while 228 were found in both extracts. The majority of the many proteins identified were not directly related to the biological activities reported here. Nevertheless, some were classified as toxins/potentially interesting molecules: lectins, proteases and protease inhibitors were detected in both extracts, while the pore-forming toxin and hyaluronidase were associated with Sp-VP. Proteolytic and anti-microbial activities were linked to both extracts, while the main toxic activities - cardiovascular, inflammatory, hemolytic and nociceptive - were elicited only by Sp-VAe. Our study provided a clear picture on the composition of the skin mucus and the venom. We also show that the classic effects observed upon envenomation are produced by molecules from the venomous gland. Our results add to the growing catalogue of scorpaeniform fish venoms and their skin mucus proteins.Significance: In this study a large number of proteins - including classical and non-classical toxins - were identified in the venomous apparatus and the skin mucus extracts of the Scorpaena plumieri fish through shotgun proteomic approach. It was shown that the toxic effects observed upon envenomation are elicited by molecules originated from the venomous gland. These results add to the growing catalogue of scorpaeniform fish venoms and their skin mucus proteins - so scarcely explored when compared to the venoms and bioactive components of terrestrial animals.Data are available via ProteomeXchange with identifier PXD009983