Microbial communities associated with filter materials in recirculating aquaculture systems of freshwater fish
Microbial communities associated with filter materials in recirculating aquaculture systems of freshwater fish
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DOI：
10.1016/j.aquaculture.2004.09.028
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发表时间：
2005-01-03
期刊：
影响因子：
4.5
通讯作者：
Shimada, T
中科院分区：
文献类型：
--
作者：
Sugita, H;Nakamura, H;Shimada, T
采用16 SrRNA基因克隆文库法对鲤鱼和金鱼封闭循环水系统中与滤料相关的微生物群落进行了研究。采用六次洗涤处理和抽汲相结合的方法可有效地回收细菌细胞。在条件良好的循环水系统中，卵石上的细菌密度在鲤鱼饲养池中为1.1 × 10（7）cells g（-1），在金鱼饲养池中为1.9 × 10（8）cells g（-1）。克隆的91个rDNA序列被α-变形菌门的成员所占据（52个克隆，属于19个分类群），β-变形菌门（14个克隆，分属8个类群），硝化螺旋菌属（Nitrospira（8个克隆属于1个分类群），放线菌（6个克隆，属于4个分类群），芽孢杆菌（3个克隆属于3个分类单元）、γ-变形菌门（3个克隆属于3个分类单元）、浮菌门（3个克隆属于3个分类单元）和鞘氨醇菌门（2个克隆属于1个分类单元）。然而，只有三个类群，这是密切相关的Hyphomicrobium spermificans，广盐红壳菌和莫斯科硝化螺菌，通常检测到鲤鱼和金鱼饲养系统。结果表明，两种系统的细菌密度和组成存在较大差异，鲤鱼池克隆文库的细菌种类多达29种，金鱼池克隆文库的细菌种类主要为丝状微菌，出现率为42%。这些结果意味着至少有36%的克隆可以利用无机氮，NH 4+，NO2-或NO3-，这表明他们关心的氮在配备了循环系统的养鱼设施的动态。(C)2004 Elsevier B. V.保留所有权利。
The microbial communities associated with filter materials in closed recirculated systems of carp and goldfish were examined by the clone library method of 16S rRNA gene. The bacterial cells were efficiently recovered by the combination of six washing treatment and swabbing. The bacterial density on pebbles of well-conditioned recirculating water systems was 1.1 x 10(7) cells g(-1) in the carp-rearing tank and 1.9 x 10(8) cells g(-1) in the goldfish-rearing aquarium. The 91 cloned rDNA sequences were occupied by members within Alphaproteobacteria (52 clones belonging to 19 taxa), Betaproteobacteria (14 clones belonging to 8 taxa), Nitrospira (8 clones belonging to 1 taxon), Actinobacteria (6 clones belonging to 4 taxa), Bacilli (3 clones belonging to 3 taxa), Gammaproteobacteria (3 clones belonging to 3 taxa), Planctomycetacia (3 clones belonging to 3 taxa) and Sphingobacteria (2 clones belonging to 1 taxon). However, only three taxa, which are closely related to Hyphomicrobium denitrificans, Rhodovulum euryhalinum and Nitrospira moscoviensis, were commonly detected in both carp- and goldfish-rearing systems. These results reveal that there are great differences in both bacterial density and composition between the two different systems, and that the clone library constructed from carp-rearing tank is characterized by as many as 29 taxa of bacteria, and that those from goldfish-rearing aquarium is characterized by Hyphomicrobium facilis with occurrence of 42%. These results mean that at least 36% of total clones could utilize the inorganic nitrogen, NH4+, NO2- or NO3-, suggesting that they concern the dynamics of nitrogen in fish-rearing facilities equipped with the recirculating system. (C) 2004 Elsevier B.V. All rights reserved.