Sex-Biased miRNA Expression in Atlantic Halibut (Hippoglossus hippoglossus) Brain and Gonads
Sex-Biased miRNA Expression in Atlantic Halibut (Hippoglossus hippoglossus) Brain and Gonads
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DOI：
10.1159/000341378
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发表时间：
2012-01-01
影响因子：
2.3
通讯作者：
Babiak, I.
中科院分区：
文献类型：
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作者：
Bizuayehu, T. T.;Babiak, J.;Babiak, I.
miRNA在鱼类性发育中的作用尚不清楚。我们使用SOLID测序技术分析了大西洋比目鱼性腺和大脑中的miRNA。我们发现了几种miRNA的组织和性别二态表达，包括miR-29 a、miR-34、miR-143、miR-145、miR-202- 3 p、miR-451和miR-2188。miR-9和miR-202分别在脑和性腺中丰富。在下一步中，我们选择了一些在性别之间表现出差异表达模式的miRNA，并对3个年龄组进行了RT-qPCR：青少年，3岁和5岁。在大脑中，与成年人相比，青少年的miR-451显著下调。let-7a、miR-143和miR-202- 3 p在相同年龄的成熟雄性性腺中的表达高于未成熟雌性。我们研究了抑制芳香化酶细胞色素P450酶对miRNA表达的影响，在性别分化的开始，通过与法倔唑或17-α-甲基睾酮雄性化。我们发现男性化个体和未处理对照之间的miRNA表达存在显著差异。与雄性青少年相比，雌性青少年中miR-202- 3 p显著下调。let-7a和miR-451的表达水平在雄性化处理终止后恢复。我们的数据首次揭示了miRNA参与硬骨鱼性发育的机制。版权所有（C）2012 S. Karger AG，巴塞尔
The role of miRNA in fish sexual development is not elucidated yet. We profiled miRNAs in gonads and brains of Atlantic halibut using SOLID sequencing technology. We found tissue- and sexually dimorphic expression of several miRNAs, including miR-29a, miR-34, miR-143, miR-145, miR-202-3p, miR-451, and miR-2188. miR-9 and miR-202 were abundant in brain and gonads, respectively. In the next step, we selected some miRNAs showing differential expression patterns between sexes and performed RT-qPCR on 3 age groups: juveniles, 3-year-, and 5-year-olds. In brains, miR-451 was significantly down-regulated in juveniles compared to adults. let-7a, miR-143, and miR-202-3p were up-regulated in gonads of mature males compared to immature females at the same age. We investigated the effect of suppressing aromatase cytochrome P450 enzyme on miRNA expression at the onset of sex differentiation through masculinization with Fadrozole or 17-alpha-methyltestosterone. We found significant differences in miRNA expression between masculinized individuals and untreated controls. miR-202-3p was significantly down-regulated in female juveniles compared to male juveniles. The expression levels of let-7a and miR-451 were restored after termination of the masculinization treatment. Our data give a first insight into miRNA involvement in sexual development in teleosts. Copyright (C) 2012 S. Karger AG, Basel