STRUCTURE AND FUNCTION OF SELECTABLE AND NON-SELECTABLE TRANSGENES IN MAIZE AFTER INTRODUCTION BY PARTICLE BOMBARDMENT
STRUCTURE AND FUNCTION OF SELECTABLE AND NON-SELECTABLE TRANSGENES IN MAIZE AFTER INTRODUCTION BY PARTICLE BOMBARDMENT
复制标题
DOI：
10.1007/bf00014669
复制
发表时间：
1994-09-01
影响因子：
5.1
通讯作者：
WILSON, HM
中科院分区：
文献类型：
--
作者：
REGISTER, JC;PETERSON, DJ;WILSON, HM
用基因枪法转化玉米A188×B73的胚性悬浮培养细胞，在卡那霉素或Bialaphos上进行筛选，对转基因整合、表达和遗传特性进行了研究。由bar或par基因介导的Bialaphos选择比由nptII基因介导的卡那霉素选择更有效。大多数转化子含有多拷贝、单基因座、转基因插入事件。如果在愈伤组织生长过程中不选择该基因的表达，转基因表达盒更有可能被重排。并不是所有代表单一转化事件的愈伤组织再生的植株都表达了转基因，并且非选择基因(UidA)在比可选择转基因更少的植株中表达。在被评估的转化子中，大约三分之二的转化子观察到了与转基因插入一致的孟德尔遗传。在后代植株中，转基因表达通常是可预测的，但并不总是如此--在一些亲本表达转基因的植物品系中，观察到转基因沉默以及转基因向后代传递较差。
Zea mays transformants produced by particle bombardment of embryogenic suspension culture cells of the genotype A188 x B73 and selected on kanamycin or bialaphos were characterized with respect to transgene integration, expression, and inheritance. Selection on bialaphos, mediated by the bar or par genes, was more efficient than selection on kanamycin, mediated by the nptII gene. Most transformants contained multicopy, single locus, transgene insertion events. A transgene expression cassette was more likely to be rearranged if expression of that gene was not selected for during callus growth. Not all plants regenerated from calli representing single transformation events expressed the transgenes, and a nonselectable gene (uidA) was expressed in fewer plants than was the selectable transgene. Mendelian inheritance of transgenes consistent with transgene insertion at a single locus was observed for approximately two thirds of the transformants assessed. Transgene expression was typically, but not always, predictable in progeny plants - transgene silencing, as well as poor transgene transmission to progeny, was observed in some plant lines in which the parent plants had expressed the transgene.