NicoLase-An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy.
NicoLase-An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy.
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DOI：
10.1371/journal.pone.0173879
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Gaus K
中科院分区：
文献类型：
--
作者：
Nicovich PR;Walsh J;Böcking T;Gaus K
现代荧光显微镜需要软件控制的照明源，在广泛的波长范围内具有高功率。半导体激光器满足功率要求，并将多个单元组合成一根光纤发射，扩展了它们在所需光谱范围内的能力。我们介绍了NicoLase，一个开源的半导体激光组合器，光纤发射，以及用于荧光显微镜和超分辨率显微镜应用的软件序列控制器。描述了两种配置，提供四个或六个输出波长和一个或两个单模光纤输出，所有CAD文件、机械师图纸和控制器源代码均公开可用。
Modern fluorescence microscopy requires software-controlled illumination sources with high power across a wide range of wavelengths. Diode lasers meet the power requirements and combining multiple units into a single fiber launch expands their capability across the required spectral range. We present the NicoLase, an open-source diode laser combiner, fiber launch, and software sequence controller for fluorescence microscopy and super-resolution microscopy applications. Two configurations are described, giving four or six output wavelengths and one or two single-mode fiber outputs, with all CAD files, machinist drawings, and controller source code openly available.