Investigation of Microbiological Methods to Estimating Individual Caries Risk: Evaluation of Sampling Methods and Materials.
Investigation of Microbiological Methods to Estimating Individual Caries Risk: Evaluation of Sampling Methods and Materials.
复制标题
评估个体龋齿风险的微生物方法研究：采样方法和材料的评估。
DOI：
10.5466/ijoms.1.29
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
K. Fukushima
中科院分区：
文献类型：
--
作者：
T. Neta;R. Inokuchi;Noriko Shinozaki‐Kuwahara;Yoshiharu Kouno;T. Ikemi;K. Fukushima
在7天的时间里，连续采集5名志愿者的唾液、拭子菌斑和刷菌斑样本4次，用选择性培养基法测定每份样本中的总链球菌(TS)和变形链球菌(MS)的数量。刷牙菌斑和刺激性唾液中MS的百分比(MS%)在一个采样期内几乎保持不变，而擦拭菌斑的TS中MS的百分比几乎保持不变，两个样本的平均MS%的比率在不同受试者之间有显著差异。这些发现表明，刷牙处理1分钟后回收的菌斑样本作为个体龋病风险评估的采样材料优于刺激的全唾液。
Stimulated-saliva, swabbing-plaque and brushing-plaque samples were successively collected from five volunteers four times over a seven-day period and the number of total streptococci (TS) and mutans streptococci (MS) in each sample were determined using selective media. The percentages of MS in TS (MS%) of the brushing-plaque and the stimulated saliva, but not the swabbing-plaque, were kept almost constant through a sampling period, and the ratio of mean MS% in both samples significantly varied among subjects. These findings suggest that plaque sample recovered by brushing treatment for 1 min is superior to stimulated whole saliva as a sampling material for assessment of individual caries risk.