Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium.
Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium.
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发表时间：
1989-11
影响因子：
4
通讯作者：
J. Taylor‐Papadimitriou;M. Stampfer;J. Bartek;A. Lewis;M. Boshell;E. Lane;I. Leigh
中科院分区：
文献类型：
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作者：
J. Taylor‐Papadimitriou;M. Stampfer;J. Bartek;A. Lewis;M. Boshell;E. Lane;I. Leigh
人乳腺的腔上皮细胞和基底上皮细胞可以根据它们表达的角蛋白的模式在组织切片上区分开来。此外，原发癌中的浸润性细胞显示出与优势腔细胞(7、8、18、19)相对应的角蛋白图谱。当来自牛奶或乳腺癌转移瘤的同质腔上皮细胞被培养时，体内所见的角蛋白表达情况保持不变。因此，我们使用与单个角蛋白反应的单特异性抗体来检测在三种不同的介质中培养的细胞的表型，这些细胞来自于同时包含腔细胞和基底细胞的缩乳术组织。在其中一种培养液(MCDB170)中培养的原发乳腺癌细胞的表型也被检测。在表征细胞表型时，还使用了针对管腔细胞在体内表达的多形性上皮粘蛋白(PEM)的抗体，以及在体内由基底细胞表达的针对平滑肌(A)肌动蛋白的抗体。我们的结果表明，不同细胞表型的增殖在不同的介质中是有选择的。在牛奶混合物(MX)中，只有腔细胞的表型才会增殖(只有1到2代)。在MCDB 170培养液中，基底层细胞增殖，而MM培养液中以基本表型为主，但也有少量具有管腔表型的细胞。在第3代左右，在MCDB 170培养液中，大部分细胞衰老，部分细胞在进一步传代时增殖。这些细胞保留了基底上皮角蛋白的表达，但也表达了管腔上皮细胞的一些特征，提示基底层可能包含一种可以沿着管腔谱系发展的干细胞。然而，在培养中，它们不表达角蛋白19，而角蛋白19在体内是完全分化的腔细胞的特征。在MCDB-170培养液中培养的乳腺癌细胞与在该培养液中培养的正常细胞具有相似的角蛋白图谱。它们不表达角蛋白19，即使原发癌的侵袭细胞在体内均匀地表达角蛋白19。侵袭性表型在其角蛋白图谱中对应于分化的腔细胞和转移性癌细胞系，不能使用支持相应正常细胞增殖的MX从原发乳腺癌中培养出来。
The luminal and basal epithelial cells in the human mammary gland can be distinguished in tissue sections on the basis of the pattern of keratins they express. Moreover, the invasive cells in primary carcinomas show a keratin profile that corresponds to that of the dominant luminal cell (7, 8, 18, 19). When homogeneous populations of luminal epithelial cells from milk or from breast cancer metastases are cultured the profile of keratin expression seen in vivo is maintained. We have therefore used monospecific antibodies reactive with individual keratins to examine the phenotype of cells cultured in three different media from reduction mammoplasty tissue that contains both luminal and basal cells. The phenotype of cells cultured from primary breast cancers in one of these media (MCDB170) has also been examined. In characterizing cell phenotypes, antibodies to a polymorphic epithelial mucin (PEM) expressed in vivo by luminal cells, and to smooth muscle (a) actin, expressed in vivo by basal cells, have also been used. Our results show that proliferation of different cell phenotypes is selected for in different media. In milk mix (MX) developed for growth of luminal cells from milk, only the luminal cell phenotype proliferates (for only 1 or 2 passages). In medium MCDB 170, which was developed for long-term growth of human mammary epithelial cells from reduction mammoplasty organoids, cells from the basal layer proliferate, while in MM medium the basal phenotype dominates, but a few cells with the luminal phenotype are found. Around passage 3, in medium MCDB 170, most cells senesce and a subpopulation of cells proliferates on further passage. These cells retain expression of the basal epithelial keratins but also express some features characteristic of luminal epithelial cells, suggesting that the basal layer may contain a stem cell that can develop along the luminal lineage. In culture, however, they do not express keratin 19, which in vivo is a feature of the fully differentiated luminal cell. The cells cultured from primary breast cancer in medium MCDB 170 have a similar keratin profile to that of the normal cells cultured in this medium. They do not express keratin 19, even though the invasive cells in primary cancers homogeneously express this keratin in vivo. The invasive phenotype, which in its keratin profile corresponds to the differentiated luminal cell and that of the metastatic cancer lines, cannot be cultured from primary breast cancers using MX, which supports proliferation of the corresponding normal cell.