Water-Solubilizing Hydrophobic ZnAglnSe/ZnS QDs with Tumor Targeted cRGD-Sulfobetaine-PIMA-Histamine Ligands via a Self Assembly Strategy for Bioimaging
Water-Solubilizing Hydrophobic ZnAglnSe/ZnS QDs with Tumor Targeted cRGD-Sulfobetaine-PIMA-Histamine Ligands via a Self Assembly Strategy for Bioimaging
复制标题
通过自组装策略将水溶性疏水性 ZnAgInSe/ZnS QD 与肿瘤靶向 cRGD-磺基甜菜碱-PIMA-组胺配体结合用于生物成像
DOI：
10.1021/acsami.6b16639
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发表时间：
2017-04-05
影响因子：
9.5
通讯作者：
Deng, Dawei
中科院分区：
文献类型：
--
作者：
Deng, Tao;Peng, Yanan;Deng, Dawei
研究量子点的有机-水相转移对于实现其在生物医学领域的广泛应用具有重要意义。在这个主题中，量子点和量子点基纳米复合材料的表面改性，尺寸控制和生物相容性是核心问题。本文中，通过配体交换和伴随的自组装过程，开发了由ZnAgInSe/ZnS（ZAISe/ZnS）QDs和磺基甜菜碱-PIMA-组胺（SPH）聚合物与α（v）β（3）整联蛋白受体环RGD（c-RGD）组成的新型高荧光肿瘤靶向QDs簇。研究发现，RGD-SPH量子点簇的结构有利于减少网状内皮系统（RES）对药物的捕获，静脉注射后有利于药物在肿瘤部位的选择性聚集，并通过肿瘤靶向性和增强的渗透性和滞留性（EPR）效应协同作用。同时，当与α（v）β（3）整联蛋白受体过表达的恶性细胞（U87 MG肿瘤）共培养时，这些簇也识别并富集细胞表面。在此基础上，制备具有长期循环和双重靶向效应的多功能纳米复合材料将成为体外和体内肿瘤成像的一个有趣策略。
Exploring the organic-to-aqueous phase transfer of quantum dots (QDs) is significant for achieving their versatile applications in biomedical fields. In this thematic issue, surface modification, size control, and biocompatibility of QDs and QDs-based nanocomposites are core problems. Herein, the new highly fluorescent tumor-targeted QDs-clusters consisting of ZnAgInSe/ZnS (ZAISe/ZnS) QDs and sulfobetaine-PIMA-histamine (SPH) polymer with the alpha(v)beta(3) integrin receptor cyclic RGD (c-RGD) were developed via ligand exchange and an accompanying self-assembly process. It was found that the structure of RGD-SPH QDs-clusters was propitious to reduce the capture of reticulo-endothelial system (RES) in virtue of external stealth ligands, and benefit to selectively accumulate at the tumor site after intravenous injection via active tumor targeting cooperated with the enhanced permeability and retention (EPR) effect. In the meantime, those clusters also recognized and enriched the cell surface when cocultured with the alpha(v)beta(3) integrin receptor overexpressed malignant cells (U87MG tumor). On the basis of the results, fabricating mutil-functional nanocomposites integrated with the long-term circulation and dual-targeting effects should be an interesting strategy for imaging cancer in vitro and in vivo.