Epidermal growth factor receptor number decreases during rat liver regeneration.
Epidermal growth factor receptor number decreases during rat liver regeneration.
复制标题
大鼠肝脏再生过程中表皮生长因子受体数量减少。
DOI：
10.1172/jci110190
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
O'Keefe,EJ
中科院分区：
文献类型：
--
作者：
Earp,HS;O'Keefe,EJ
为探讨表皮生长因子(EGF)对大鼠肝部分切除后不同时间分离的肝膜的结合作用，探讨EGF在大鼠肝再生调控中的作用。结果表明，肝部分切除后8h即可检测到~(125)I-EGF结合力下降。肝部分切除后36和48h，EGF结合量降至最低点，低于假手术对照组的40%。Scatchard分析表明，结合能力的下降是由于受体数量的下降。在125I-胰岛素和125I-麦芽凝集素结合的平行研究中证实了观察到的EGF受体丢失的特异性；在肝再生期间，这些配体的结合没有明显减少。这些数据与EGF或类似物质是调节肝脏再生的复杂体液信号的一个成分的假设一致。
The potential role of epidermal growth factor (EGF) in the regulation of rat liver regeneration was examined by assessing the binding of 125I-EGF to hepatic membranes isolated at various times after partial hepatectomy. The results demonstrated a fall in 125I-EGF binding detectable as early as 8 h after partial hepatectomy. The nadir in EGF binding, less than 40% of that observed in sham-operated control rats, was seen 36 and 48 h after partial hepatectomy. Scatchard analysis showed that the decrease in binding capacity was due to a fall in receptor number. The specificity of the observed loss of EGF receptors was substantiated in parallel studies of 125I-insulin and 125I-wheat germ lectin binding; the binding of these ligands did not decrease appreciably during liver regeneration. The data are consistent with the hypothesis that EGF or a similar substance is one component of the complex humoral signal that regulates liver regeneration.