Clarification of the nucleotide sequence at the 5'-end of the cDNA for rat liver carnitine palmitoyltransferase II.
Clarification of the nucleotide sequence at the 5'-end of the cDNA for rat liver carnitine palmitoyltransferase II.
复制标题
大鼠肝肉毒碱棕榈酰转移酶 II cDNA 5 端核苷酸序列的澄清。
DOI：
10.1042/bj2960271
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
McGarry,JD
中科院分区：
文献类型：
--
作者：
Weis,BC;Foster,DW;McGarry,JD
围绕大鼠肝脏肉毒碱棕榈酰转移酶 II (CPT II) 对应的 cDNA 结构引起了争议。该问题源于 Brady 等人的报告 [1, 2]，其中建议我们发表的序列 [3] 在 5' 端缺少相当长的核苷酸，因此预测蛋白质缺少天然产物的 N 端。我们之前已经以多种理由反驳了这一论点 (3-6]。特别令我们困惑的是，在 [21 Brady 等人显然分离出了一个大鼠 CPT II 基因组克隆，该克隆在我们指定为翻译起始位点的 ATG 密码子 5' 的核苷酸序列方面与我们的 cDNA 有很大不同(图 1)。为了支持该材料的真实性，Brady 等人[2]指出它包含一个核苷酸序列（图 1 中的 B-1） lb) 编码八肽，他们认为该八肽代表了成熟蛋白的真正 N 末端。出于这些原因，我们必须考虑这样的可能性：我们的 cDNA 毕竟在其 5' 端较短，或者，大鼠肝脏包含与所讨论的两个序列相对应的 CPT II 转录本（图 1a 和 1b）。 因此，从正常大鼠和用邻苯二甲酸二乙酯 (DEHP；过氧化物酶体和线粒体酶的诱导剂[8]）。
Controversy has arisen surrounding the structure of the cDNA corresponding to rat liver carnitine palmitoyltransferase II (CPT II). The problem stems from reports by Brady et al.[1, 2] in which it was suggested that our published sequence [3] lacked a considerable stretch of nucleotides at the 5'-end, and thus predicted a protein lacking the N-terminus of the natural product. We have previously countered this argument on a variety of grounds (3-6]. Particularly puzzling to us was that in [21 Brady et al. had apparently isolated a rat CPT II genomic clone that differed substantially from our cDNA in terms of its nucleotide sequence 5'to the ATG codon that we had assigned as the translation start site (Figure 1). In support of the authenticity of this material, Brady et al.[2] noted that it contained a nucleotide sequence (B-1 in Figure lb) encoding an octapeptide whichthey believe to representthe true N-terminus of the mature protein. For-these reasons we had to entertain the possibility that our cDNA was, after all, short at its 5'end or, alternatively, that rat liver contains CPT II transcripts corresponding to both of the sequences in question (Figures la and lb). Accordingly, poly (A)+ RNA was isolated from livers of normal rats and of animals treated with diethylhexylphthalate (DEHP; an inducer of peroxi-somal and mitochondrial enzymes [8]). From this, first-strand (a)