Identification of hispidin as a bioluminescent active compound and its recycling biosynthesis in the luminous fungal fruiting body
Identification of hispidin as a bioluminescent active compound and its recycling biosynthesis in the luminous fungal fruiting body
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DOI：
10.1039/c7pp00216e
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发表时间：
2017-09-01
影响因子：
3.1
通讯作者：
Stevani, Cassius V.
中科院分区：
文献类型：
--
作者：
Oba, Yuichi;Suzuki, Yoshiki;Stevani, Cassius V.
我们以前表明，发光真菌共享一个共同的机制，在生物发光，并确定hispidin作为前体的Neonothopanus nambi菌丝体。在这里，我们表明，hispidin作为一种生物发光活性化合物在25-1000 pmol g（-1）的小菇chlorophos的子实体中，Omnitus gardneri，和Neonothopanus gardneri的存在。这些结果表明，发光蘑菇含有hispidin作为前体。我们还发现，不发光的“年轻”子实体表现出发光的hispidin治疗。此外，我们观察到通过添加hispidin生物合成组分，即咖啡酸，ATP和丙二酰辅酶A，无细胞子实体提取物的逐渐发光增强。这些发现表明发光蘑菇的持续微弱发光是由hispidin的缓慢循环生物合成调节的。
We previously showed that luminous fungi share a common mechanism in bioluminescence, and identified hispidin as a luciferin precursor in Neonothopanus nambi mycelium. Here we showed the presence of hispidin as a bioluminescent active compound at 25-1000 pmol g(-1) in the fruiting bodies of Mycena chlorophos, Omphalotus japonicus, and Neonothopanus gardneri. These results suggest that luminous mushrooms contain hispidin as a luciferin precursor. We also found that non-luminous "young" fruiting bodies exhibited luminescence by hispidin treatment. Furthermore, we observed a gradual luminescence enhancement of the cell-free fruiting body extract by the addition of hispidin biosynthetic components, namely caffeic acid, ATP and malonyl-CoA. These findings suggest that continuous weak glow of luminous mushrooms is regulated by slow recycling biosynthesis of hispidin.