Different progress of MDCK cell death after infection by two different influenza virus isolates
Different progress of MDCK cell death after infection by two different influenza virus isolates
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DOI：
10.1002/(sici)1099-0844(19970601)15:2
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发表时间：
1997-06
影响因子：
3.6
通讯作者：
Zuzana Horníčková
中科院分区：
文献类型：
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作者：
Zuzana Horníčková
研究了 1994 年和 1995 年捷克共和国分离的 A 型 (H3N2) 和 B 型流感病毒株对 MDCK 细胞的影响。在细胞培养过程中使用不同浓度的病毒和营养条件。在存在和不存在 10% 小牛血清的情况下，研究了病毒复制、基因组 DNA 片段化以及细胞毒性。与组织培养中未感染的细胞相比，病毒复制，无论是 A 型还是 B 型，都会导致更早的 DNA 断裂。结果表明，乙型流感病毒株对MDCK细胞的细胞毒作用较甲型流感病毒更大。甲型流感病毒感染剂量较高，细胞凋亡加速；相反，较高的乙型流感病毒感染剂量会延迟细胞凋亡的发生。在体外条件下，血清的缺乏增强了流感诱导的细胞凋亡的进展。 © 1997 约翰威利父子公司。
The effect of influenza strains A (H3N2) and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with cytotoxicity were investigated in the absence and presence of 10 per cent calf serum. Virus replication, regardless of type A or B, caused earlier DNA fragmentation in comparison to non‐infected cells in tissue culture. The results showed that the influenza B strain had a greater cytotoxic effect on MDCK cells than influenza A. A higher infection dose of influenza A virus accelerated the onset of apoptosis; conversely, a higher infection dose of influenza B virus delayed the onset of apoptosis. The absence of serum enhanced the progress of influenza‐induced apoptosis in conditions in vitro. © 1997 John Wiley & Sons, Ltd.