A New Strategy for Developing an Animal Model of Temporal Lobe Epilepsy
A New Strategy for Developing an Animal Model of Temporal Lobe Epilepsy
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DOI：
10.4172/neuropsychiatry.1000530
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Yuanxin Huang;Guofeng Wu;Si-ying Ren;Yangting Kang;Jing Wu
中科院分区：
文献类型：
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作者：
Yuanxin Huang;Guofeng Wu;Si-ying Ren;Yangting Kang;Jing Wu
目的探索一种新的颞叶癫痫动物模型制作方法。方法选用160只健康成年雄性SD大鼠，采用3种不同的方法建立颞叶癫痫模型，筛选最佳方法建立颞叶癫痫模型。实验分为杏仁核点燃对照组（AKC组）、氯化锂-匹罗卡品对照组（LPC组）、氯化锂-匹罗卡品+杏仁核点燃组（PAK组）。其余健康正常大鼠作为正常大鼠对照组（NRC组）。比较各组大鼠癫痫发作次数、脑电图改变、ZnT 3、胶质纤维酸性蛋白（GFAP）、GABAA受体表达及尼氏染色检测海马锥体细胞数。结果PAK组颞叶癫痫造模成功率、自发性癫痫发作率均明显高于AKC组。与LPC组相比，PAK组的死亡率显著降低。PAK、AKC和LPC组癫痫大鼠海马锥体细胞和GABAA受体数量减少，MFS和星形胶质细胞增生。与传统杏仁核点燃模型相比，腹腔注射氯化锂-匹罗卡品联合杏仁核点燃（PAK组）可缩短造模时间，提高颞叶癫痫造模成功率。与传统的氯化锂-匹罗卡品模型相比，改良模型（PAK组）的死亡率显著降低。改良模型（PAK组）与杏仁核点燃模型（AKC组）和氯化锂-匹罗卡品模型（LPC组）的病理特征一致。这是一种可行的改进颞叶癫痫大鼠模型的方法。结论本研究所采用的颞叶癫痫模型制作策略是一种可靠的方法。与单一杏仁核点燃相比，该策略可提高制备颞叶癫痫模型的成功率，与单一氯化锂-匹罗卡品模型相比，可降低死亡率。
Objective To develop a new strategy for generating an animal model of temporal lobe epilepsy. Methods A total of 160 healthy adult male SD rats were used to generate the models of temporal lobe epilepsy by three kinds of techniques and select an optimal technique to generate a suitable model of temporal lobe epilepsy. The animals were assigned to a amygdale-kindling control group (AKC group), lithium chloride-pilocarpine control group (LPC group), a lithium chloride-pilocarpine followed by amygdale-kindling group (PAK group). Other healthy normal rats were used as normal rat control group (NRC group). The epileptic seizures, the EEG changes, the expression of ZnT3, Glial Fibrillary Acidic Protein (GFAP), and GABAA receptors, the number of hippocampal pyramidal cells detected by Nissl’s staining were compared among groups. Results The rate of successful model of temporal lobe epilepsy, the spontaneous seizures in the PAK group were significantly greater than those in the AKC group. The mortality in the PAK group was significantly decreased compared to the LPC group. The decreased number of pyramidal cells and GABAA receptors, MFS and astrocyte hyperplasia were found in the hippocampus of the epileptic rats in the PAK, AKC and LPC groups. Compared with the traditional amygdala kindling model, intraperitoneal injection of lithium chloride-pilocarpine combined with amygdala kindling (PAK group) decreased the time of modeling, increased the success rate of temporal lobe epilepsy modeling. Compared with the traditional lithium chloride-pilocarpine model, the mortality decreased significantly in the improved model (PAK group). The pathological features of the reformative model (PAK group) were consistent with the results of the amygdala kindling model (AKC group) and lithium chloride-pilocarpine model(LPC group). It is a viable way to improve the rat model of temporal lobe epilepsy. Conclusions The strategies for developing an temporal lobe epilepsy model in our study might be a reliable method. Such strategies increased the success rate of preparing the model of temporal lobe epilepsy than single amygadale kindling and decreased the mortality compared with the single lithium chloride-pilocarpine model.