Acute and long-term effects of a single dose of the fungicide carbendazim (methyl 2-benzimidazole carbamate) on the male reproductive system in the rat.
Acute and long-term effects of a single dose of the fungicide carbendazim (methyl 2-benzimidazole carbamate) on the male reproductive system in the rat.
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DOI：
10.1002/j.1939-4640.1992.tb00345.x
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发表时间：
1992-11
影响因子：
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通讯作者：
Masaaki Nakai;Rex A. Hess;Billy J. Moore;Robert F. Guttroff;L. Strader;Ralph E. Linder
中科院分区：
文献类型：
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作者：
Masaaki Nakai;Rex A. Hess;Billy J. Moore;Robert F. Guttroff;L. Strader;Ralph E. Linder
在成年大鼠单次口服剂量后测定多菌灵（2-苯并咪唑氨基甲酸甲酯）对睾丸、传出小管和精子的影响。进行了两项实验：使用 0 和 400 mg/kg 剂量在暴露后 2 小时到 32 天之间进行时间响应，以及使用 0 到 800 mg/kg 剂量在暴露后 2 和 70 天进行剂量响应。在实验 1 中，从暴露后 8 小时开始，整个 32 天期间都可以看到影响；其影响首先包括睾丸重量增加，然后睾丸精子细胞数量和形态正常尾部精子的百分比减少。在实验2中，用100mg/kg或更高剂量治疗的动物发生了显着的睾丸和传出导管改变。治疗后2天睾丸重量呈剂量依赖性增加，同时伴随着生精管直径的增加和未成熟生殖细胞以阶段依赖性方式的过度损失。传出小管闭塞的发生率也呈剂量依赖性增加。闭塞的导管的特点是严重炎症并表现出上皮的紊乱。 70 天时，平均睾丸重量和平均生精管直径出现剂量依赖性下降；然而，50 mg/kg 剂量时仅观察到极小的长期影响。在表现出大于 25%（100 mg/kg 或更大剂量）的生精小管萎缩的睾丸中，所有睾丸均与包含闭塞的传出小管相关。这些睾丸中的精子数量显着减少。在导管传出管中观察到闭塞、异常导管、纤维化、精索肉芽肿和矿化。多菌灵对睾丸的长期影响主要是由导管闭塞引起的。结果表明，多菌灵对男性生殖系统产生比杀菌剂苯菌灵更严重的短期和长期影响。
The effects of carbendazim (methyl 2-benzimidazole carbamate) on the testis, efferent ductules, and sperm were determined in the adult rat after a single oral dose. Two experimental trials were performed: a time response between 2 hours and 32 days after exposure using 0 and 400 mg/kg, and a dose response at 2 and 70 days after exposure using 0 to 800 mg/kg doses. In experiment 1, effects were seen throughout the 32-day period, beginning 8 hours after exposure; the effects included first an increase in testis weight, then decreases in testicular spermatid numbers and in the percentage of morphologically normal cauda sperm. In experiment 2, significant testicular and efferent ductal alterations occurred in animals treated with doses of 100 mg/kg or greater. A dose-dependent increase in testicular weight 2 days after treatment was accompanied by increases in seminiferous tubular diameter and excessive loss of immature germ cells in a stage-dependent manner. There was also a dose-dependent increased incidence of occlusions in the efferent ductules. The occluded ductules were characterized by severe inflammation and exhibited disorganization of the epithelium. At 70 days, there were dose-dependent decreases in mean testis weight and mean seminiferous tubular diameter; however, only minimal long-term effects were seen at 50 mg/kg. In testes exhibiting seminiferous tubular atrophy of greater than 25% (100 mg/kg or greater doses), all of the testes were associated with efferent ductules containing occlusions. Caput sperm numbers were significantly reduced in these testes. Occlusions, abnormal ductules, fibrosis, spermatic granulomas, and mineralization were observed in the ductuli efferents. Long-term effects of carbendazim on the testis were induced primarily by ductal occlusions. Results show that carbendazim produces more severe short- and long-term effects on the male reproductive system than the fungicide benomyl.