Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
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DOI：
10.3389/fncel.2020.00063
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发表时间：
2020-03-19
影响因子：
5.3
通讯作者：
Shigemoto, Ryuichi
中科院分区：
文献类型：
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作者：
Eguchi, Kohgaku;Velicky, Philipp;Shigemoto, Ryuichi
急性脑片制备是研究脑内突触功能特征的一种有效的实验模型。虽然脑组织通常在冰冷的温度（CT）下切割以便于切片并避免神经元损伤，但暴露于CT会导致突触的分子和结构变化。为了解决这些问题，我们研究了在冰冷和生理温度（PT）下制备的小鼠急性小脑切片中突触的超微结构和电生理特征。在CT制备的切片中，我们发现了显著的棘丢失和重建、突触囊泡重排和突触蛋白减少，所有这些在PT制备的切片中均未检测到。与这些结构发现一致，在PT制备的切片显示出更高的释放概率。此外，准备在PT允许电生理记录后立即切片导致更高的检测能力的长期抑郁症（LTD）运动学习后相比，在CT。这些结果表明，在PT切片制备研究突触功能在不同的生理条件下的实质性优势。
Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural changes of synapses. To address these issues, we investigated ultrastructural and electrophysiological features of synapses in mouse acute cerebellar slices prepared at ice-cold and physiological temperature (PT). In the slices prepared at CT, we found significant spine loss and reconstruction, synaptic vesicle rearrangement and decrease in synaptic proteins, all of which were not detected in slices prepared at PT. Consistent with these structural findings, slices prepared at PT showed higher release probability. Furthermore, preparation at PT allows electrophysiological recording immediately after slicing resulting in higher detectability of long-term depression (LTD) after motor learning compared with that at CT. These results indicate substantial advantages of the slice preparation at PT for investigating synaptic functions in different physiological conditions.