The copper chelator methanobactin from Methylosinus trichosporium OB3b binds copper(I)
The copper chelator methanobactin from Methylosinus trichosporium OB3b binds copper(I)
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DOI：
10.1021/ja0558140
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发表时间：
2005-12-14
影响因子：
15
通讯作者：
Rosenzweig, AC
中科院分区：
文献类型：
--
作者：
Hakemian, AS;Tinberg, CE;Rosenzweig, AC
研究了铜与甲烷菌（methanobactin）结合的氧化态。甲烷菌是甲烷营养物methylosinustrichosporiumob3b中的一种类似铁载体的小分子。负载Cu（II）的纯化甲烷菌素表现出较弱的EPR信号，可能是由于Cu（II）的外源性。加入强氧化剂硝酸后，EPR信号强度显著增加。x射线吸收近边光谱的特征，包括在8985 eV处的1s→4p跃迁，进一步表明Cu(I)的存在。EXAFS数据最适合使用由先前报道的晶体学参数产生的多重散射模型。这些结果明确地确立了m。trichosporiumOB3b methanobactin与Cu(I)结合，表明methanobactin自身将Cu（II）还原为Cu(I)。
The oxidation state of copper bound to methanobactin, a small siderophore-like molecule from the methanotrophMethylosinustrichosporiumOB3b, was investigated. Purified methanobactin loaded with Cu(II) exhibits a weak EPR signal probably due to adventitious Cu(II). The EPR signal intensity increases significantly upon addition of the strong oxidant nitric acid. Features of the X-ray absorption near edge spectrum, including a 1s → 4p transition at 8985 eV, further indicate the presence of Cu(I). EXAFS data were best fit using a multiple scattering model generated from previously reported crystallographic parameters. These results establish definitively thatM.trichosporiumOB3b methanobactin binds Cu(I) and suggest that methanobactin itself reduces Cu(II) to Cu(I).