Preliminary characterization of the normal microbiota of the human vulva using cultivation-independent methods
Preliminary characterization of the normal microbiota of the human vulva using cultivation-independent methods
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DOI：
10.1099/jmm.0.46607-0
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发表时间：
2007-02-01
影响因子：
3
通讯作者：
Forney, Larry J.
中科院分区：
文献类型：
--
作者：
Brown, Celeste J.;Wong, Mayee;Forney, Larry J.
本研究的目的是对正常人外阴的微生物种群进行初步表征。从大阴唇样品中分离基因组DNA。和阴唇。minora从四个健康的妇女，并在每个细菌16S rRNA基因序列进行了测定。将这些序列与已知细菌物种的序列进行比较，以对这些群落中数量丰富的种群进行分类。即使在这有限数量的个体中，人类外阴的微生物群也被发现是相当多样化的。每个女性都有独特的微生物群，没有一个单一的物种是所有女性共同的。微生物群。大阴唇和小阴唇中的乳酸杆菌和严格厌氧菌的数量不同，但两者都有相当数量的乳酸杆菌和严格厌氧菌。与小阴唇相比，大阴唇的种群多样性更大。结果表明，外阴的微生物群包括已知的皮肤，结肠和阴道微生物群的分支，并且比以前认为的要复杂得多，这表明需要进行更广泛的研究。
The objective of this study was to perform a preliminary characterization of the microbial populations of the normal human vulva. Genomic DNA was isolated from samples of the labia majora. and labia. minora from four healthy women, and sequences of bacterial 16S rRNA genes in each were determined. The sequences were compared with those of known bacterial species to classify the numerically abundant populations in these communities. Even among this limited number of individuals, the microbiota of the human vulva was found to be quite diverse. Each woman had a distinctive microbiota and no single species was common to all women. The microbiota. of the labia majora and labia minora differed, although both had appreciable numbers of lactobacilli and strict anaerobes. A greater diversity of populations inhabited the labia majora compared with the labia minora. The results indicated that the microbiota of the vulva includes populations known to be commensals of the microbiota of the skin, colon and vagina, and is much more complex than previously thought, suggesting that more extensive investigations are warranted.