Template directed incorporation of nucleotide mixtures using azole-nucleobase analogs.
Template directed incorporation of nucleotide mixtures using azole-nucleobase analogs.
复制标题
使用唑核碱基类似物模板定向掺入核苷酸混合物。
DOI：
10.1093/nar/25.24.4866
复制
发表时间：
1997
影响因子：
14.9
通讯作者：
Davisson,VJ
中科院分区：
文献类型：
--
作者：
Hoops,GC;Zhang,P;Johnson,WT;Paul,N;Bergstrom,DE;Davisson,VJ
DNA that encodes elements for degenerate replication events by use of artificial nucleobases offers a versatile approach to manipulating sequences for applications in biotechnology. We have designed a family of artificial nucleobases that are capable of assuming multiple hydrogen bonding orientations through internal bond rotations to provide a means for degenerate molecular recognition. Incorporation of these analogs into a single position of a PCR primer allowed for analysis of their template effects on DNA amplification catalyzed byThermus aquaticus(Taq) DNA polymerase. All of the nucleobase surrogates have similar shapes but differ by structural alterations that influence their electronic character. These subtle distinctions were able to influence theTaqDNA polymerase dependent incorporation of the four natural deoxyribonucleotides and thus, significantly expand the molecular design possibilities for biochemically functional nucleic acid analogs.
