Primary sequence differences between Chido and Rodgers variants of tryptic C4d of the human complement system
Primary sequence differences between Chido and Rodgers variants of tryptic C4d of the human complement system
复制标题
人类补体系统胰蛋白酶 C4d 的 Chido 和 Rodgers 变体之间的主要序列差异
DOI：
10.1016/0014-5793(84)81323-x
复制
发表时间：
1984
期刊：
影响因子：
3.5
通讯作者：
J. Sjöquist
中科院分区：
文献类型：
--
作者：
U. Hellman;G. Eggertsen;Å. Lundwall;Åke Engström;J. Sjöquist
用溴化氰和胰蛋白酶将Chido和Rodgers变异体的人胰蛋白酶C4d片段化。通过氨基酸分析和序列测定对片段进行了表征。在5个位点上检测到两种遗传变异体之间的多态性。其中4个位点（第141、142、145、146位残基）位置相近，分别为Leu、Ser、Ile、His和Pro、Cys、Leu、Asp。在位置94中，在Chido中发现Gly，在Rodgers中发现Asp。对片段进行比对，得出结论，两种变体的胰蛋白酶C4d均含有346个残基。
Human tryptic C4d of the Chido and Rodgers variant was fragmented by cyanogen bromide and trypsin. The fragments were characterized by amino acid analysis and sequence determination. Polymorphism between the two genetic variants was detected in 5 positions. Four were closely located (residues 141, 142, 145, 146), where Leu, Ser, Ile, His occurred in the Chido variant and Pro, Cys, Leu, Asp in the Rodgers variant, respectively. In position 94 Gly was found in Chido and Asp in Rodgers. Alignment of the fragments was performed and it is concluded that tryptic C4d of both variants contains 346 residues.