Schistosoma: Rate of glucose import is altered by acetylcholine interaction with tegumental acetylcholine receptors and acetylcholinesterase
Schistosoma: Rate of glucose import is altered by acetylcholine interaction with tegumental acetylcholine receptors and acetylcholinesterase
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DOI：
10.1006/expr.1995.1152
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发表时间：
1995-12-01
影响因子：
2.1
通讯作者：
Agnew, A
中科院分区：
文献类型：
--
作者：
Camacho, M;Agnew, A
在血栖期的棘体被膜上有乙酰胆碱酯酶（AChE）和烟碱样乙酰胆碱受体（nAChR）。AChE和nAChR都集中在成年雄性的背侧表面，这是蠕虫对营养吸收的主要表面。暴露于乙酰胆碱（ACh），这些分子的天然配体的皮层AChE和nAChR，有一个后果，在某些物种的这种膜的运输功能。血吸虫和牛血吸虫成虫对葡萄糖的摄取率在体外增强约60%，在血液浓度的乙酰胆碱。曼氏血吸虫没有表现出类似的反应。乙酰胆碱与nAChR和AChE相互作用的特异性通过用nAChR的特异性拮抗剂（d-筒箭毒碱和α-银环蛇毒素）和AChE的抑制剂（BW 284 C51）的作用的消融来显示。由于α-银环蛇毒素和BW 284 C51不能穿透蛇体皮层，因此主要作用发生在皮层上。依赖这种机制的物种差异与它们对乙酰胆碱酯酶抑制药物美曲磷酯的相对敏感性一致。(C)出版社：Academic Press
The blood dwelling stages of schistosomes have acetylcholinesterase (AChE) and nicotinic-like acetylcholine receptors (nAChR) on their teguments. Both AChE and nAChR are concentrated on the dorsal surface of the adult male, a major surface for nutrient uptake for the worm pair. Exposure of tegumental AChE and nAChR to acetylcholine (ACh), the natural ligand of these molecules, has a consequence for the transporting function of this membrane in some schistosome species. The rate of glucose uptake in vitro by Schistosoma haematobium and Schistosoma bovis adult worm pairs was enhanced by approximately 60% at blood concentrations of ACh. Schistosoma mansoni did not show a similar response. The specificity of the ACh interaction with nAChR and AChE was shown by ablation of the effect with specific antagonists of nAChR (d-tubocurarine and alpha-bungarotoxin) and an inhibitor of AChE (BW284C51). The primary effect occurs on the tegument since alpha-bungarotoxin and BW284C51 do not penetrate the schistosome tegument. The species differences in reliance on this mechanism are consistent with their relative sensitivities to the AChE inhibitory drug, metrifonate. (C) 1995 Academic Press, Inc.