Effect of size and shape on toxicity of zinc oxide (ZnO) nanomaterials in human peripheral blood lymphocytes
Effect of size and shape on toxicity of zinc oxide (ZnO) nanomaterials in human peripheral blood lymphocytes
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DOI：
10.1080/15376516.2017.1366609
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发表时间：
2018-02
影响因子：
3.2
通讯作者：
D. Shalini;S. Senthilkumar;P. Rajaguru
中科院分区：
文献类型：
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作者：
D. Shalini;S. Senthilkumar;P. Rajaguru
摘要氧化锌纳米材料（ZnO NMs）的多工业应用导致人类暴露增加。虽然ZnO纳米颗粒（NPs）的毒性以前已经进行了评估，但其他形式的ZnO纳米材料的毒性尚未进行评估。在这项研究中，四种不同类型的ZnO纳米材料的细胞毒性和遗传毒性进行了评估，使用人外周血淋巴细胞（HPBL）。此外，还评价了抗氧化剂对ZnO纳米材料诱导的毒性的影响。我们的研究结果表明，纳米材料的尺寸和形状对其毒性有深远的影响。纳米粒子和纳米棒（NRs）比微粒（MP）和微棒（MR）具有更高的氧化电位和ROS产生能力。与此相反，MPs和MRs具有更高水平的脂质过氧化能力。较小的NP更具有遗传毒性，而较大的MP和MR本质上更具细胞毒性。用维生素C或槲皮素治疗显著降低了与ZnO NM相关的遗传毒性。纳米材料的大小和形状对纳米材料毒性的影响应予以考虑，可能的抗氧化剂的补充可能会减轻纳米材料的毒性。
Abstract The multi-industrial applications of zinc oxide nanomaterials (ZnO NMs) lead to increasing exposure to humans. Though the ZnO nanoparticles (NPs) toxicity had been evaluated previously, toxicity of other forms of ZnO nanomaterials has not been evaluated. In this study, cytotoxicity and genotoxicity of four different types of ZnO NMs were evaluated using human peripheral blood lymphocytes (HPBL). In addition, the effect of anti-oxidants on ZnO NMs induced toxicity was also evaluated. Our results suggest that, size and shape of the nanomaterials have profound effects on their toxicity. The NPs and nanorods (NRs) possessed higher level of oxidative potential and ROS generation capacity than microparticles (MPs) and microrods (MRs). In contrast, MPs and MRs possessed higher level of lipid peroxidation capacity. The smaller NPs are more genotoxic while larger MPs and MRs were more cytotoxic in nature. Treatment with vitamin C or Quercetin significantly reduces the genotoxicity associated with ZnO NMs. The influence of size and shape in mediating NMs toxicity should be taken into account and the possible supplementation of anti-oxidants might mitigate the toxicity.