Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex
Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex
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DOI：
10.1016/j.bbaexp.2005.09.005
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发表时间：
2005-11-10
期刊：
影响因子：
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通讯作者：
Workman, JL
中科院分区：
文献类型：
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作者：
Carrozza, MJ;Florens, L;Workman, JL
酿酒酵母Rpd3的组蛋白去乙酰化抑制由Ash1和Ume6 DNA结合蛋白调控的基因Rpd3存在于小的0.6MDa（Rpd3S）和大的1.2MDa（Rpd3L）辅阻遏物复合物中。在这份报告中，我们确定质谱和MudPIT的Rpd3L复合物的亚基。这些包括Rpd3、Sds3、Pho23、Dep1、Rxt2、Sin3、Ash1、Ume1、Sap30、Cti6、Rxt3和Ume6。Rpd3L的独特组分Dep1和Sds3是Rpd3L完整性和HDAC活性所必需的。与RPD3类似，DEP1的缺失增强了端粒沉默并解除了INO1的抑制。两个序列特异性阻遏物Ash1和Ume6与Rpd3L稳定相关。虽然这两种蛋白定位于INO1和HO1启动子，但这些基因的抑制分别仅依赖于Ume6和Ash1。因此，Rpd3L复合物通过多个整合的DNA结合蛋白直接募集到特异性启动子。(c)2005 Elsevier B.V.保留所有权利。
Histone deacetylation by Saccharomyces cerevisiae Rpd3 represses genes regulated by the Ash1 and Ume6 DNA-binding proteins. Rpd3 exists in a small 0.6 MDa (Rpd3S) and large 1.2 MDa (Rpd3L) corepressor complex. In this report, we identify by mass spectrometry and MudPIT the subunits of the Rpd3L complex. These included Rpd3, Sds3, Pho23, Dep1, Rxt2, Sin3, Ash1, Ume1, Sap30, Cti6, Rxt3 and Ume6. Dep1 and Sds3, unique components of Rpd3L, were required for Rpd3L integrity and HDAC activity. Similar to RPD3, deletion of DEP1 enhanced telomeric silencing and derepressed INO1. Two sequence-specific repressors, Ash1 and Ume6, were stably associated with Rpd3L. While both of these proteins localized to the INO1 and HO1 promoters, the repression of these genes were dependent only on Ume6 and Ash1, respectively. Thus, the Rpd3L complex is directly recruited to specific promoters through multiple integral DNA-binding proteins. (c) 2005 Elsevier B.V. All rights reserved.