Detection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry
Detection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry
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DOI：
10.1002/rcm.3355
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Bingham, Sheila A.
中科院分区：
文献类型：
--
作者：
Kuhnle, Gunter G. C.;Joosen, Annemiek M. C. P.;Bingham, Sheila A.
一些流行病学研究表明，精制糖的摄入量与结直肠癌、乳腺癌、胰腺癌和子宫内膜癌的风险增加之间存在联系。然而，其他研究未能证实这些结论，其原因可能是主要采用自我报告的饮食评估方法的模糊性。蔗糖是糖摄入量的既定生物标志物，可以客观评估膳食蔗糖。到目前为止，蔗糖的尿排泄主要使用酶测定法测定。然而，这种方法是耗时和劳动密集型的。在这项研究中，我们提出了一种质谱法测定蔗糖在尿液中使用液相色谱质谱（LC/MS），可用于大规模的流行病学研究。版权所有（c）2008约翰威利父子有限公司。
Several epidemiological studies suggest a link between the intake of refined sugars and an increased risk for colorectal, breast, pancreatic and endometrial cancer. However, other studies failed to confirm these conclusions and the reason for this may be the ambiguity of dietary assessment methods mainly self-reporting - employed. Sucrose is an established biomarker for sugars intake, allowing the objective assessment of dietary sucrose. So far, urinary excretion of sucrose was mainly determined using an enzyme assay. However, this method is time-consuming and labour-intensive. In this study, we present a mass spectrometric method for the determination of sucrose in urine using liquid chromatography with mass spectrometry (LC/MS) which can be used for large-scale epidemiological studies. Copyright (c) 2008 John Wiley & Sons, Ltd.