Dual-wavelength digital holographic phase and fluorescence microscopy for an optical thickness encoded suspension array
Dual-wavelength digital holographic phase and fluorescence microscopy for an optical thickness encoded suspension array
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用于光学厚度编码悬浮阵列的双波长数字全息相位和荧光显微镜
DOI：
10.1364/ol.43.000739
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发表时间：
2018-02-15
期刊：
影响因子：
3.6
通讯作者：
Ji, Yanhong
中科院分区：
文献类型：
--
作者：
Shen, Zhiyuan;He, Yonghong;Ji, Yanhong
设计了一套双波长数字全息相位和荧光显微系统，作为光学厚度编码悬浮阵列的解码和检测平台。相位成像路径用于解码光学厚度编码的微载体，荧光成像路径用于检测结合的目标分析物的定量信息。编码容量可以超过100。通过多重免疫分析实验验证了相位成像路径的解码可靠性。通过浓度梯度实验证实了荧光成像路径的定量分析能力。该方法提供了高解码精度和高检测灵敏度的标记信号。(C)2018美国光学学会
A dual-wavelength digital holographic phase and fluorescence microscopy system is demonstrated as the decoding and detection platform of an optical thickness encoded suspension array. The phase imaging path is designed to decode optical thickness encoded microcarriers, and the fluorescence imaging path is used to detect the quantitative information of the bound target analytes. The encoding capacity could be more than 100. The decoding reliability of the phase imaging path is verified by a multiplexed immunoassay experiment. The ability for quantitative analysis of the fluorescence imaging path is confirmed by concentration gradient experiments. This method offers high decoding accuracy and high detection sensitivity of label signals. (C) 2018 Optical Society of America