Ultrastructural studies on the corneal superficial epithelium of rats by in vivo cryofixation with freeze substitution.
Ultrastructural studies on the corneal superficial epithelium of rats by in vivo cryofixation with freeze substitution.
复制标题
通过冷冻替代体内冷冻固定对大鼠角膜浅层上皮进行超微结构研究。
DOI：
10.1159/000267738
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发表时间：
1995
影响因子：
2.1
通讯作者：
S. Tsukahara
中科院分区：
文献类型：
--
作者：
H. B. Chen;S. Yamabayashi;B. Ou;S. Ohno;S. Tsukahara
本文应用活体冷冻置换固定法（VC-FS），在透射电镜下观察了正常人角膜表面上皮的超微结构，并与常规方法进行了比较。用VC-FS方法，超微结构深至8微米保存完好的标本。角膜表面有一层均匀的物质，被认为是泪膜。除了第一层上皮细胞下的细胞间隙，有时也是第二层上皮细胞下的细胞间隙外，其他相邻细胞紧密接触，它们之间或桥粒之间没有任何间隙。相比之下，当使用常规方法时，在上皮的所有层中的相邻细胞之间发现细胞间隙，并且在角膜表面上没有泪膜残留。VC-FS被认为是一种比传统方法更好地保存组织结构的自然状态的方法，并且是用于角膜上皮和泪膜的形态学研究的有用方法。细胞间隙是一种伪影，通过这种方法可以减少。
The method of in vivo cryofixation with freeze substitution (VC-FS) was used to examine the natural ultrastructure of the corneal superficial epithelium under the transmission electron microscope, and the results have been compared with the conventional method. With the VC-FS method, the ultrastructure down to 8 microns in depth was well preserved in the specimen. There was a layer of homogeneous material thought to be tear film on the corneal surface. Except for the intercellular spaces presenting under the first and sometimes also the second layers of the epithelial cells, other adjacent cells were in close contact without any spaces between them or between the desmosomes. In contrast, when using the conventional method, intercellular spaces were found between adjacent cells in all the layers of epithelium, and no tear film remained on the corneal surface. VC-FS is considered to be a better way to preserve the structure of tissue in its natural state than the conventional method and to be a useful method for the morphological study of the corneal epithelium and the tear film. Intercellular spaces, a kind of artifact, can be reduced by this method.