Extreme Expression of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Breast Cancer As Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry
Extreme Expression of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Breast Cancer As Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry
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DOI：
10.1021/acs.biochem.5b00928
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发表时间：
2015-09-29
期刊：
影响因子：
2.9
通讯作者：
Dizdaroglu, Miral
中科院分区：
文献类型：
--
作者：
Coskun, Erdem;Jaruga, Pawel;Dizdaroglu, Miral
脱嘌呤/脱嘧啶核酸内切酶1（Apurinic/apyrimidinic endonuclease 1，APE 1）是一种DNA修复蛋白，具有重要的生物学功能。据报道，癌症中的APE 1水平升高。然而，测量APE 1水平的现有方法是间接的，而不是定量的。我们以前开发了一种方法，使用液相色谱和串联质谱与同位素稀释，以准确地测量APE 1水平。在这里，我们应用这种方法来测量正常和癌性人类乳腺组织中的APE 1水平。在恶性肿瘤中观察到APE 1的极端表达，表明乳腺癌细胞可能需要AIDE 1才能生存。准确测量APE 1对于开发新的治疗策略和APE 1抑制剂作为抗癌药物可能是必不可少的。
Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem mass spectrometry with isotope dilution to accurately measure APE1 levels. Here, we applied this methodology to measure APE1 levels in normal and cancerous human breast tissues. Extreme expression of APE1 in malignant tumors was observed, suggesting that breast cancer cells may require AIDE1 for survival. Accurate measurement of APE1 may be essential for the development of novel treatment strategies and APE1 inhibitors as anticancer drugs.